1. Absence of Microsatellite Instability and Lack of Evidence for Subclone Diversification in the Pathogenesis and Progression of Mycosis Fungoides 
Chalid Assaf, José A.A. Sanchez, Ansgar Lukowsky, Konrad Kölble, Tanja Fischer, Pierluigi Amerio, Wolfram Sterry, Peter Walden 
Journal of Investigative Dermatology 
Volume 127, Issue 7, Pages (July 2007)
DOI: /sj.jid
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Clinical, histological, and molecular evaluation of lesion of MF patient 12 in the clinical course of disease. (a–d) Patch/plaque stage at initial presentation and (e–h) tumor stage. (a) Erythematous patches and plaques in the left abdominal region, (b) corresponding histology showing a broad dermal infiltrate and epidermotropic small lymphocytes (original magnification: 100μm), (c) Genescan profile of clonal TCRγ rearrangement (252bp), and (d) Genescan profile of one exemplary microsatellite pattern (DXS453) showing the MS fragment size of 159bp. (e) Multiple erythematous tumor nodules at the back and chest. (f) Histology revealing Pautrier's microabscesses with large atypical lymphocytes and heavy dermal infiltration (original magnification: 50μm), (g) Genescan profile of the TCRγ PCR documenting the presence of the same T-cell clone as in the initial lesion (252bp), and (h) Genescan microsatellite pattern (DXS453) that reproduces the pattern found for the earlier lesion with a microsatellite fragment size of 159bp.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Profiles of the four autosomal microsatellites in control peripheral blood cell samples and MF lesions. Representative PCR Genescan profiles of different microsatellite loci of matched normal (above) and tumor (below) DNA from patients 4, 10, and 12.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Expression of mismatch repair proteins in mycosis fungoides. Double immunofluorescence staining with hMLH1 and CD3 showing coexpression of hMLH1 (red) and CD3 (green) in the tumor cells (sheets of tumor cells surrounding a hair follicle) in a skin lesion of the mycosis fungoides patient 12 at tumor stage analyzed by confocal microscopy (original magnification: 25μm).
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions