Presentation is loading. Please wait.

Presentation is loading. Please wait.

Qutayba Hamid, MD, PhD  Journal of Allergy and Clinical Immunology 

Published byAustin Wells Modified over 5 years ago

Similar presentations

Presentation on theme: "Qutayba Hamid, MD, PhD  Journal of Allergy and Clinical Immunology "— Presentation transcript:

1 Effects of steroids on inflammation and cytokine gene expression in airway inflammation 
Qutayba Hamid, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 112, Issue 3, Pages (September 2003) DOI: /S (03)

2 FIG 1 Inflammatory cells before and after treatment with corticosteroids in airway inflammation. There is a decrease in the number of eosinophils, mast cells, and basophils. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

3 FIG 2-4 Biopsy specimens from the upper airways show an increase in the inflammatory cells, particularly eosinophils (Figs 2 and 3), which decrease significantly after treatment with steroids (Fig 4). Note also the squamous metaplasia (Fig 2) and the detachment of the epithelium (Fig 3). Although not in all cases, prolonged treatment with steroids might restore normal phenotype (Fig 4). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

4 FIG 2-4 Biopsy specimens from the upper airways show an increase in the inflammatory cells, particularly eosinophils (Figs 2 and 3), which decrease significantly after treatment with steroids (Fig 4). Note also the squamous metaplasia (Fig 2) and the detachment of the epithelium (Fig 3). Although not in all cases, prolonged treatment with steroids might restore normal phenotype (Fig 4). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

5 FIG 2-4 Biopsy specimens from the upper airways show an increase in the inflammatory cells, particularly eosinophils (Figs 2 and 3), which decrease significantly after treatment with steroids (Fig 4). Note also the squamous metaplasia (Fig 2) and the detachment of the epithelium (Fig 3). Although not in all cases, prolonged treatment with steroids might restore normal phenotype (Fig 4). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

6 FIG 5-6 Eosinophils could be detected through use of a noninvasive method in sputum preparation (Fig 5); treatment with corticosteroids is associated with a decrease in the number of eosinophils in sputum (Fig 6). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

7 FIG 5-6 Eosinophils could be detected through use of a noninvasive method in sputum preparation (Fig 5); treatment with corticosteroids is associated with a decrease in the number of eosinophils in sputum (Fig 6). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

8 FIG 7 The effect of steroids on cytokine gene expression in steroid-sensitive patients. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

9 FIG 8-9 Inability of steroids to inhibit cytokine gene expression in asthmatic individuals showing a high level of IL-2 and IL-4 expression in steroid-resistant asthma (Fig 8). This could lead to upregulation of GRβ (Fig 9), which is detected in bronchoalveolar lavage preparation. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

10 FIG 8-9 Inability of steroids to inhibit cytokine gene expression in asthmatic individuals showing a high level of IL-2 and IL-4 expression in steroid-resistant asthma (Fig 8). This could lead to upregulation of GRβ (Fig 9), which is detected in bronchoalveolar lavage preparation. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

11 FIG 10-11 Effect of corticosteroids on chemokine gene expression (Fig 10), with an example of MCP-4 immunoreactivity in the epithelium before and after steroids (Fig 11). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

12 FIG 10-11 Effect of corticosteroids on chemokine gene expression (Fig 10), with an example of MCP-4 immunoreactivity in the epithelium before and after steroids (Fig 11). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

13 FIG 12-13 Steroids have no or little effect on subepithelial fibrosis or smooth muscle mass. Shown are a collagen staining in endobronchial biopsy after prolonged treatment with inhaled steroids (Fig 12) and a Masson Trichrome staining of an endobronchial biopsy specimen from an asthmatic patient after steroid treatment (Fig 13), illustrating persistent increase in subepithelial fibrosis and an increase in smooth muscle mass. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

14 FIG 12-13 Steroids have no or little effect on subepithelial fibrosis or smooth muscle mass. Shown are a collagen staining in endobronchial biopsy after prolonged treatment with inhaled steroids (Fig 12) and a Masson Trichrome staining of an endobronchial biopsy specimen from an asthmatic patient after steroid treatment (Fig 13), illustrating persistent increase in subepithelial fibrosis and an increase in smooth muscle mass. Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

15 FIG 14-15 Inhaled steroids (HFA preparation) have the ability to be deposited in peripheral airways, where they can decrease eosinophil numbers (Fig 14) as well as the expression of eotaxin and IL-5 (Fig 15). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

16 FIG 14-15 Inhaled steroids (HFA preparation) have the ability to be deposited in peripheral airways, where they can decrease eosinophil numbers (Fig 14) as well as the expression of eotaxin and IL-5 (Fig 15). Journal of Allergy and Clinical Immunology  , DOI: ( /S (03) )

Download ppt "Qutayba Hamid, MD, PhD  Journal of Allergy and Clinical Immunology "

Similar presentations

Inhaled corticosteroids decrease subepithelial collagen deposition by modulation of the balance between matrix metalloproteinase-9 and tissue inhibitor.

Inhaled corticosteroids decrease subepithelial collagen deposition by modulation of the balance between matrix metalloproteinase-9 and tissue inhibitor.
What effect does asthma treatment have on airway remodeling

What effect does asthma treatment have on airway remodeling
Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.
Bronchial subepithelial fibrosis and expression of matrix metalloproteinase-9 in asthmatic airway inflammation  Makoto Hoshino, MD, Yutaka Nakamura, MD,

Bronchial subepithelial fibrosis and expression of matrix metalloproteinase-9 in asthmatic airway inflammation  Makoto Hoshino, MD, Yutaka Nakamura, MD,
Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma  Makoto Hoshino, MDa, Yutaka Nakamura, MDa,

Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma  Makoto Hoshino, MDa, Yutaka Nakamura, MDa,
The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,
Obstructive sleep apnea syndrome and inflammation

Obstructive sleep apnea syndrome and inflammation
Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,

Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,
Role of airway smooth muscle in airway remodeling

Role of airway smooth muscle in airway remodeling
Airway epithelial cells in asthma

Airway epithelial cells in asthma
Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.
Increased TGF-β2 in severe asthma with eosinophilia

Increased TGF-β2 in severe asthma with eosinophilia
Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug- induced cutaneous eruptions  Nikhil Yawalkar, MD, Maithili Shrikhande,

Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug- induced cutaneous eruptions  Nikhil Yawalkar, MD, Maithili Shrikhande,
Role of IL-9 in the pathophysiology of allergic diseases

Role of IL-9 in the pathophysiology of allergic diseases
Airway remodeling in subjects with severe asthma with or without chronic persistent airflow obstruction  Marta Kaminska, MD, Susan Foley, MD, Karim Maghni,

Airway remodeling in subjects with severe asthma with or without chronic persistent airflow obstruction  Marta Kaminska, MD, Susan Foley, MD, Karim Maghni,
Peter J. Barnes, FMedSci, FRS 

Peter J. Barnes, FMedSci, FRS 
Jennifer L. Ingram, PhD, Monica Kraft, MD 

Jennifer L. Ingram, PhD, Monica Kraft, MD 
Allergic airway disease is unaffected by the absence of IL-4Rα–dependent alternatively activated macrophages  Natalie E. Nieuwenhuizen, PhD, Frank Kirstein,

Allergic airway disease is unaffected by the absence of IL-4Rα–dependent alternatively activated macrophages  Natalie E. Nieuwenhuizen, PhD, Frank Kirstein,
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
CD4+ T cells migrate from airway to bone marrow after antigen inhalation in rats  Susumu Isogai, MD, Shigeru Miyata, MD, Rame Taha, MD, Yasuyuki Yoshizawa,

CD4+ T cells migrate from airway to bone marrow after antigen inhalation in rats  Susumu Isogai, MD, Shigeru Miyata, MD, Rame Taha, MD, Yasuyuki Yoshizawa,

Similar presentations

Ads by Google