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Improved development of mouse and human embryos using a tilting embryo culture system  Koji Matsuura, Nobuyoshi Hayashi, Yuka Kuroda, Chisato Takiue,

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Presentation on theme: "Improved development of mouse and human embryos using a tilting embryo culture system  Koji Matsuura, Nobuyoshi Hayashi, Yuka Kuroda, Chisato Takiue,"— Presentation transcript:

1 Improved development of mouse and human embryos using a tilting embryo culture system 
Koji Matsuura, Nobuyoshi Hayashi, Yuka Kuroda, Chisato Takiue, Rei Hirata, Mami Takenami, Yoko Aoi, Nanako Yoshioka, Toshihiro Habara, Tetsunori Mukaida, Keiji Naruse  Reproductive BioMedicine Online  Volume 20, Issue 3, Pages (March 2010) DOI: /j.rbmo Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 (A) The tilting embryo culture system, (B) diagram of the motion programme and (C) time–dish angle graph and definition of the dish angle. Vr, radial velocity (degree/s); θr, maximum tilt angle (degree); Th, holding time at the maximum tilt angle (min). The ranges of the three parameters are 0.1–1degree/s, 1–20degrees and 1–60min, respectively. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Motions of mouse embryos by tilting microscope, showing: (A and B) movement before and after tilting and (C and D) before and after holding the tilt. Arrows in B=movement in 6s; arrows in D=movement in 40s. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

4 Figure 3 Relationship of the ratio of the number of mouse embryos to the volume of media and mouse blastocyst development rates. Light and dark grey bars indicate the number of blastocysts of the control and tilting embryo culture system groups, respectively. Error bars are SEM. ∗Statistically significant differences (P<0.05). Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

5 Figure 4 Comparison of average cell numbers of mouse blastocysts under the culture condition of 4–6 embryos in a 50μl microdrop between the control (CTRL) and tilting embryo culture system (TECS) groups. Error bars are SEM. ∗Statistically significant differences (P<0.05). Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

6 Figure 5 Comparison of average cell numbers of human blastocysts between the control (CTRL) and tilting embryo culture system (TECS) groups. Error bars are SEM. Statistically significant difference is indicated: ∗P<0.05. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

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