1. H3K36 Methylation Is Involved in Promoting Rice Flowering
Pengfei Sui, Jinlei Shi, Xueying Gao, Wen-Hui Shen, Aiwu Dong 
Molecular Plant 
Volume 6, Issue 3, Pages (May 2013)
DOI: /mp/sss152
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
The Rice SDG725-Knockdown Mutant 725Ri-1 Shows Late-Flowering Phenotype, Reduced Expression of Several Positive Regulatory Flowering Genes, and Decrease in H3K36me2/3 Levels at Chromatin of the Regulated Genes.
APhenotype comparison of 78-day-old wild-type (left) and 725Ri-1 mutant (right) rice plants grown under long-day photoperiods. Bar = 30 cm.
BHeading time of wild-type (light column) and 725Ri-1 mutant (dark column) rice plants grown under long-day (LD, Shanghai field) or short-day (SD, Sanya field) photoperiods. Error bars indicate SD (n = 30).
CSchematic representation of gene regulatory pathways involved in rice flowering-time control.
DRelative mRNA levels of different flowering regulatory genes determined by quantitative RT–PCR. Analyses were performed using leaves collected after 2 h dawn from 45-day-old rice plants grown in a growth chamber under long-day photoperiods (14-h light/10-h dark). Expression level of flowering genes was normalized to Ubiquitin5 and the fold change of 725Ri-1 mutant (dark column) compared to wild-type (light column, set at 1) is shown. Bars indicate SD from three independent repeat experiments.
ERelative levels of H3K36me1, H3K36me2, H3K36me3, H3K4me2, H3K4me3, and SDG725 at chromatin of flowering genes determined by ChIP analysis. Two regions (–1 and –2) of each gene were analyzed. Actin 1 gene was used as internal control. Each column (light for wild-type and dark for 725Ri-1) represents the mean value together with SD bar from three independent repeat experiments.
Statistical analysis revealed that differences between the 725Ri-1 mutant and wild-type are significant at P < 0.05 (two-sided t-test) in all cases, except where indicated by (–), the differences are not significant.
Molecular Plant 2013 6, DOI: ( /mp/sss152)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions