Presentation is loading. Please wait.

Presentation is loading. Please wait.

Mechanisms underlying differential food allergy response to heated egg

Published byEmery Stanley Modified over 6 years ago

Similar presentations

Presentation on theme: "Mechanisms underlying differential food allergy response to heated egg"— Presentation transcript:

1 Mechanisms underlying differential food allergy response to heated egg
Gustavo Martos, BS, Ivan Lopez-Exposito, PhD, Ramon Bencharitiwong, BS, M. Cecilia Berin, PhD, Anna Nowak-Węgrzyn, MD  Journal of Allergy and Clinical Immunology  Volume 127, Issue 4, Pages e2 (April 2011) DOI: /j.jaci Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, Mice body temperature on oral challenge with native (N) or heated (H) OVA or ovomucoid (OM). B, Serum OVA-specific and OM-specific IgE levels of the different mice groups before challenge. OD values correspond to 1/100 serum dilution. ∗Statistically significant differences between native and heated challenged groups; P < .001. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Immunoblotting of unheated or heated (H) ovomucoid (OM) and OVA by using pooled sera from heated egg–reactive children. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 RP-HPLC chromatograms corresponding to native (N) and heated (H) OVA and ovomucoid (OM) undigested and subjected to gastric and gastroduodenal digestion. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Percentage of activated basophils (CD63+CD123+CD203c+HLA-DRlow) on stimulation with native, heated (H), and/or digested (Dig) OVA or ovomucoid (OM). A, Detailed activated basophil population from 1 donor. B, Activation percentages from 3 donors. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 NHR of RBL cells passively sensitized with ovomucoid (OM)–reactive mice’s sera and stimulated with different concentrations (Conc.) of undigested/digested native (N) and heated (H) OM. NHR release by negative controls (digestive enzymes, buffer) was not significantly different from spontaneous release, which was subtracted in the final graph. Positive control, anti-IgE stimulation, was 60%. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Percentage of activated basophils (CD63+CD123+CD203c+HLA-DRlow) on stimulation with native or heated (H) OVA or ovomucoid (OM) transcytosed by Caco-2 cells. A, Detailed activated basophil population from 1 experiment. B, Mean activation percentages from 3 replicates. Error bars represent SD. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig 7 OVA-specific T-cell activation in the gastrointestinal lymphoid tissue. CFSE-labeled DO11.10 T cells were transferred to naive BALB/c mice before gavage feeding with 25 mg OVA or heated (H) OVA. After 72 hours, proliferation of DO11.10 T cells was assessed by flow cytometry by using cells isolated from MLN or PP. Data are shown from 2 individual OVA-fed or H-OVA–fed mice. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 In vitro IL-13 and IFN-γ induction on stimulation with OVA or ovomucoid (OM) in cultured spleen cells from OVA-sensitized (A) or OM-sensitized (B) mice challenged with native (dotted bars) or heated (striped bars) OVA or OM. Conc., Concentration. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 SDS-PAGE of unheated (A) and heated (B) OVA and of unheated (C) and heated (D) ovomucoid, subjected to pepsin digestion for 0, 1, 5, 10, 30, and 60 minutes (lanes 1-6, respectively). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Mechanisms underlying differential food allergy response to heated egg"

Similar presentations

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,
Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.

Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.
Shea butter contains no IgE-binding soluble proteins

Shea butter contains no IgE-binding soluble proteins
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H
IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.
Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.

Alternation of circadian clock modulates forkhead box protein-3 gene transcription in CD4+ T cells in the intestine  Gui Yang, MD, Hong Zhang, PhD, Yi.
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A
IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.
Ann-Marie M. Schoos, MD, PhD, Jacob D

Ann-Marie M. Schoos, MD, PhD, Jacob D
Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 

Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.

Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.

Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.
Ting-ting Zhang, MSc, Klaus Okkenhaug, PhD, Baher F

Ting-ting Zhang, MSc, Klaus Okkenhaug, PhD, Baher F
Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,
Insulin-like growth factor 2 enhances regulatory T-cell functions and suppresses food allergy in an experimental model  Gui Yang, MD, Xiao-Rui Geng, MD,

Insulin-like growth factor 2 enhances regulatory T-cell functions and suppresses food allergy in an experimental model  Gui Yang, MD, Xiao-Rui Geng, MD,

Similar presentations

Ads by Google