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Reciprocal interaction of the conjunctiva and cornea in ocular allergy

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Presentation on theme: "Reciprocal interaction of the conjunctiva and cornea in ocular allergy"— Presentation transcript:

1 Reciprocal interaction of the conjunctiva and cornea in ocular allergy
Ken Fukuda, MD, PhD, Teruo Nishida, MD, DSc  Journal of Allergy and Clinical Immunology  Volume 125, Issue 2, Pages e2 (February 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Effects of corneal epithelial ablation and antigen challenge on symptoms and eosinophil infiltration into the conjunctiva. Clinical score (A) and the number of conjunctival eosinophils (C) after antigen challenge with (closed bars) or without (open bars) corneal epithelial ablation in sensitized or nonsensitized rats. B, Sections of the conjunctiva from sensitized rats at 24 hours after antigen challenge with or without corneal epithelial ablation were stained with Congo red. ∗P < .05, ∗∗P < .01 versus the corresponding value for nonsensitized rats; †P < .05, ††P < .01 (Tukey-Kramer test). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Effect of allergic inflammation in the conjunctiva on corneal epithelial wound healing. Fluorescein staining of corneal epithelial defects at 48 hours (A) and time course of wound healing (B) after removal of the corneal epithelium and antigen challenge in nonsensitized (open circles) or sensitized (closed circles) rats. ∗P < .05, ∗∗P < .01 versus the corresponding value for nonsensitized rats (Tukey-Kramer test). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Effects of corneal epithelial ablation and antigen challenge on the abundance of chemokine and adhesion molecule mRNAs in the conjunctiva. Sensitized rats were challenged with PBS or RW in the presence (closed bars) or absence (open bars) of corneal epithelial ablation. The abundance of CCL11, CCL5, and intercellular adhesion molecule 1 (ICAM-1) mRNAs in the conjunctiva at 18 hours after challenge was determined by reverse transcriptase polymerase chain reaction (RT-PCR) analysis as described previously.E1 In brief, total RNA was extracted from conjunctival tissue with the use of an RNeasy Mini Kit (Qiagen, Tokyo, Japan) and was subjected to RT, and the resulting cDNA was then subjected to real-time PCR analysis with specific primers (Qiagen) and with the use of a LightCycler instrument (Roche Molecular Biochemicals, Mannheim, Germany). Transcripts of the constitutively expressed gene for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served to normalize the amount of target mRNA in each sample. Data are means ± SEMs from 5 eyes. ∗P < .05 versus the corresponding value for PBS-challenged rats (Turkey-Kramer multiple comparisons test). Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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