1. Volume 55, Issue 2, Pages 674-685 (February 1999)
Nature and mediators of renal lesions in kidney transplant patients given cyclosporine for more than one year 
Ariela Benigni, Isabella Bruzzi, Marilena Mister, Nadia Azzollini, Flavio Gaspari, Norberto Perico, Eliana Gotti, Tullio Bertani, Giuseppe Remuzzi 
Kidney International 
Volume 55, Issue 2, Pages (February 1999)
DOI: /j x
Copyright © 1999 International Society of Nephrology Terms and Conditions

2. Figure 1
Photomicrograph showing a representative distribution of endothelin-1 (ET-1) mRNA by in situ hybridization in kidney sections from a patient with cyclosporine A (CsA) nephrotoxicity at renal biopsy. Intense staining was observed in tubular epithelial cells (a, magnification ×90; b, ×360; and c, ×180). Negative control using ET-1 sense probe in an adjacent kidney section is shown (d, ×180).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

3. Figure 2
Frequency of positive tubular staining for (A) endothelin-1 (ET-1), (B) RANTES (regulated upon activation, normal T cell expressed and secreted), and (C) monocyte chemoattractant protein-1 (MCP-1) mRNAs in the kidney allograft biopsies from the three groups of transplanted recipients. Staining was considered positive when the score staining intensity was ≥1 in two or more tubuli. The numbers indicate positive staining/total biopsies analyzed.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

4. Figure 3
Representative RANTES mRNA distribution by in situ hybridization in the kidney from a patient with CsA nephrotoxicity. Intense staining was found in tubuli (a, magnification ×360), whereas weak staining in parietal epithelial cells of the glomerulus was observed (c, ×360). Strong staining was observed in interstitial inflammatory cells (e, ×360). Negative control was obtained by hybridization with a sense probe (b, d, f, ×360).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

5. Figure 4
Staining for MCP-1 mRNA by in situ hybridization in the kidney from a patient with CsA nephrotoxicity. Staining for MCP-1 mRNA was mild either in tubuli (a, magnification ×180; b, magnification ×360) as well as in glomeruli (d, magnification ×360). No signal was detected in the negative control hybridized with a sense probe (c and e, magnification ×360).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

6. Kidney International 1999 55, 674-685DOI: (10. 1046/j. 1523-1755. 1999
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

7. Kidney International 1999 55, 674-685DOI: (10. 1046/j. 1523-1755. 1999
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

8. Kidney International 1999 55, 674-685DOI: (10. 1046/j. 1523-1755. 1999
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

9. Kidney International 1999 55, 674-685DOI: (10. 1046/j. 1523-1755. 1999
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions