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Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.

Published byIrwan Iskandar Modified over 5 years ago

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Presentation on theme: "Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three."— Presentation transcript:

1 Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three injection replicates for diphosphopeptide, YHGHpS293MSDPGVpS300YR, with precursor ion M extracted at m/z Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA.A, relative quantitation over three injection replicates for diphosphopeptide, YHGHpS293MSDPGVpS300YR, with precursor ion M extracted at m/z B, relative quantitation for the corresponding phosphopeptides, YHGHpS293MSDPGVSYR and/or YHGHSMSDPGVpS300YR, with precursor ion M extracted at m/z C, phosphopeptide YGMGTpS232VER with precursor ion M extracted at m/z D, independent Western blot analysis of PDHE1α phosphorylation with phosphosite-specific and total protein antibodies in response to DCA treatment. Birgit Schilling et al. Mol Cell Proteomics 2012;11: © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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