1. The temporary site to download BALBES:
Recent Developments and Activities of BALBES Fei Long Chemistry Department, University of York
The temporary site to download BALBES:

2. Update the database and test BALBES:
Maintain BALBES:
Bug Fixing:
One major bug involves the tool “wget”. The solution is changing “wget” to “curl”
Feedback: A few new structures have been solved
Update the database and test BALBES:
Every half month, we check and take the newly deposited structures in RCSB, and then update our database.
We use these new structures (if structural factor files are also released) to test BALBES, and analyse the results, in particular, the cases that failed to give solutions.

3. Test Case Statistics Between 01 Jan -31Jan 2007
Between 01 Feb -15 Feb 2007
Between 16 Feb -28 Feb 2007
Between 01 Mar -15 Mar 2007

4. New Developments implemented
BALBES is put into our group new web server (P.Young), which is released currently only to YSBL for trial
New sequence-model search protocol implemented. It takes longer time but improved target model search
Allow an input file containing multiple sequences
New protocol that assembles domains from different structures as a search model.

5. For the structure 2 in sequence 1
| | |
| PDB_CODE | v4p |
| NO. of MODEL | |
| | |
| Model | Chain ID | Similarity | Residues | Multimer? | Domain? | Monomers(expected)|
| | | | | | | |
| | A | | | Monomer | No | |
| | A | | | Monomer | Yes | |
| | | | | | | |
| | A | | | Monomer | Yes | |
| | AB | | | Dimer | No | |
| | | | | | | |
| | ABC | | | Tremer | No | |
| | | | | | | |

6. For the structure 4 in sequence 1
| | |
| PDB_CODE | MDOM |
| NO. of MODEL| |
| Model | Chain ID | Similarity | Residues | Multimer? | Domain? | Monomers(expected)|
| | | | | | | |
| | A | | | Monomer | Yes | |
| | A | | | Monomer | Yes | |
| | A | | | Monomer | Yes | |
| | | | | | | |
| | A | | | Monomer | Yes | |

7. Assessment of the results. For each test case, we now keep a record
ono Resolution: R: R_free: MR_Score: MR_Score_pre: B_aver_all: B_aver_main: B_aver_side: Chain ScoreC B_aver_all B_aver_main B_aver_side A B C D E F G H Chain expected: 8 Chain found And try to analyse them later on using multivariate analysis method, e.g. discriminate analysis, to hopeful give better assessment of results.

8. Acknowledgements Garib Murshdov - YSBL, York
Alexei A Vagin - YSBL, York
Andrey Lebedev - YSBL, York
Poung Young YSBL, York
E.Krissinel for PISA - MSD/EBI, Cambridge
All CCP4 and YSBL people for support
Protein Data Bank depositors and maintainers for a fantastic resource
Wellcome Trust, BBSRC, EU BIOXHIT, NIH for support