Presentation is loading. Please wait.

Presentation is loading. Please wait.

Prathap Pillai, MD, Cailong Fang, PhD, Yih-Chih Chan, PhD, Mohamed H

Published byEsther Campos Modified over 5 years ago

Similar presentations

Presentation on theme: "Prathap Pillai, MD, Cailong Fang, PhD, Yih-Chih Chan, PhD, Mohamed H"— Presentation transcript:

1 Allergen-specific IgE is not detectable in the bronchial mucosa of nonatopic asthmatic patients 
Prathap Pillai, MD, Cailong Fang, PhD, Yih-Chih Chan, PhD, Mohamed H. Shamji, PhD, Clare Harper, PhD, Shih-Ying Wu, MSc, Line Ohm-Laursen, PhD, Stephen R. Durham, MD, FRCP, Andrew Menzies-Gow, MD, Raj K. Rajakulasingam, MD, Sun Ying, PhD, Chris J. Corrigan, PhD, Hannah J. Gould, PhD  Journal of Allergy and Clinical Immunology  Volume 133, Issue 6, Pages e11 (June 2014) DOI: /j.jaci Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Box and whisker plots summarizing total IgE concentrations in the serum (left) and bronchial mucosa (right) of nonatopic control subjects (NAC), atopic asthmatic patients (AA), and nonatopic asthmatic patients (NAA); Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Immunofluorescence image of a bronchial biopsy specimen from a nonatopic asthmatic patient stained for plasma cells (CD138; green), IgE (red), and nucleus (blue). Top, View of the whole biopsy specimen showing area of magnification. Bottom, Magnified view with a merged image and individual stains. Arrows indicate cells with dual staining (plasma cells expressing IgE). Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Total IgE concentrations in the serum (left y-axes, open bars) and bronchial mucosa (right y-axes, shaded bars) of atopic asthmatic patients (top), nonatopic asthmatic patients (middle), and nonatopic control subjects (bottom). Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Scatter plots with actual data points summarizing total IgE concentrations in the serum (left) and bronchial mucosa (right) of nonatopic control subjects (NAC), atopic asthmatic patients (AA), and nonatopic asthmatic patients (NAA). Median values for individual data sets are also shown (Mann-Whitney U test). Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Concentrations of IgE specific for grass (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, Phl p 11, and Cyn d 1) and tree (Bet v 1, Ole e 1, Cry j 1, Cup a 1, and Pla a 2) allergen components are expressed as percentages of total IgE in the sera (open bars) and bronchial mucosa (solid bars) of atopic asthmatic patients. Subjects sensitized to the corresponding allergens, as determined by using skin prick tests/ImmunoCAP, are indicated by arrowheads. (Figs E3 to E5 show only positive data obtained from the standard ISAC assay. In some of the atopic subjects sensitized to particular allergens by using conventional criteria [skin prick tests or PhadiaCAP], IgE against none of the corresponding allergen components in the standard ISAC chip was detectable in the serum or the bronchial mucosa. Subjects were sensitized to the following: grass—subjects 3, 4, and 8; tree—subjects 2 and 10; molds—subjects 3, 4, and 6; cat—subject 4; and dog—subject 10.) Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 Concentrations of IgE specific for HDM (Der p 1, Der p 2, Der f 1, Der f 2, and Eur m 2) and mold (Asp f 1, Asp f 3, Asp f 6, and Alt a 1) allergen components are expressed as percentages of total IgE in the sera (open bars) and bronchial mucosa (solid bars) of atopic asthmatic patients. Subjects sensitized to the corresponding allergens, as determined by using skin prick tests/ImmunoCAP, are indicated by arrowheads. Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E5 Concentrations of IgE specific for cat (Fel d 1, Fel d 2, and Fel d 4) and dog (Can f 1, Can f 2, and Can f 3) allergen components are expressed as percentages of total IgE in the sera (open bars) and bronchial mucosa (solid bars) of atopic asthmatic patients. Subjects sensitized to the corresponding allergens, as determined by using skin prick tests/ImmunoCAP, are indicated by arrowheads. Journal of Allergy and Clinical Immunology  , e11DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Prathap Pillai, MD, Cailong Fang, PhD, Yih-Chih Chan, PhD, Mohamed H"

Similar presentations

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.
Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms  Anna Slovick, MRCS, Abdel Douiri, PhD,

Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms  Anna Slovick, MRCS, Abdel Douiri, PhD,
Novel severe wheezy young children phenotypes: Boys atopic multiple-trigger and girls nonatopic uncontrolled wheeze  Jocelyne Just, PhD, MD, Rahele Gouvis-Echraghi,

Novel severe wheezy young children phenotypes: Boys atopic multiple-trigger and girls nonatopic uncontrolled wheeze  Jocelyne Just, PhD, MD, Rahele Gouvis-Echraghi,
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study  Anna.

Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study  Anna.
Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.
Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H
Chlamydophila (Chlamydia) pneumoniae serology and asthma in adults: A longitudinal analysis  Rafael Pasternack, MD, PhD, Heini Huhtala, MSc, Jussi Karjalainen,

Chlamydophila (Chlamydia) pneumoniae serology and asthma in adults: A longitudinal analysis  Rafael Pasternack, MD, PhD, Heini Huhtala, MSc, Jussi Karjalainen,
Tolerant beekeepers display venom-specific functional IgG4 antibodies in the absence of specific IgE  Eva-Maria Varga, MD, Fahima Kausar, MSc, Werner.

Tolerant beekeepers display venom-specific functional IgG4 antibodies in the absence of specific IgE  Eva-Maria Varga, MD, Fahima Kausar, MSc, Werner.
Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A
Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 

Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 
Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis  Kaori Mukai, PhD, Nicolas Gaudenzio,

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis  Kaori Mukai, PhD, Nicolas Gaudenzio,
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,
Julie Wang, MD, James H. Godbold, PhD, Hugh A. Sampson, MD 

Julie Wang, MD, James H. Godbold, PhD, Hugh A. Sampson, MD 
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study  Anna.

Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study  Anna.
Suzana Radulovic, MD, Mikila R. Jacobson, PhD, Stephen R

Suzana Radulovic, MD, Mikila R. Jacobson, PhD, Stephen R
Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses  Chris J. Corrigan, MD, PhD, Wei.
In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing  Nikolaos Douladiris, MD,

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing  Nikolaos Douladiris, MD,
Component-resolved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins∗  Ingrid Sander, PhD, Hans-Peter Rihs, PhD,

Component-resolved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins∗  Ingrid Sander, PhD, Hans-Peter Rihs, PhD,

Similar presentations

Ads by Google