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Identification of bee venom Api m 1 IgE epitopes and characterization of corresponding mimotopes  Abida Zahirović, MPharm, Ana Koren, PhD, Peter Kopač,

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Presentation on theme: "Identification of bee venom Api m 1 IgE epitopes and characterization of corresponding mimotopes  Abida Zahirović, MPharm, Ana Koren, PhD, Peter Kopač,"— Presentation transcript:

1 Identification of bee venom Api m 1 IgE epitopes and characterization of corresponding mimotopes 
Abida Zahirović, MPharm, Ana Koren, PhD, Peter Kopač, MD, Borut Štrukelj, PhD, Peter Korošec, PhD, Mojca Lunder, PhD  Journal of Allergy and Clinical Immunology  Volume 143, Issue 2, Pages e5 (February 2019) DOI: /j.jaci Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 The peptide sequences obtained by biopanning of phage display libraries with anti–Api m 1 IgG. A, Peptides carrying consensus motif GP[S/N/D]ELG[R/K/H] were aligned to the N-terminal region of Api m 1. B, Peptides carrying D[K/R/H]SKP(H) consensus motif were aligned to the C-terminal region of Api m 1. Amino acids in red (A) and green (B) are the same or have similar physicochemical properties as residues in Api m 1 primary sequence. C, The surface locations of the 2 epitopes (red and green) identified by computational mapping of mimotopes to the 3-dimensional structure of Api m 1 are presented. D, Binding of nApi m 1 and pIII-fused peptides to IgE from sera of bee venom–allergic patients. Increasing concentration of nApi m 1 (1-4). Peptides matching to Api m 1 epitope between residues 17 and 24: pIII-SPPNALGRFLPD (5), pIII-LMGPSEL (6), and pIII-CWTDLGRKC (7). Peptide matching to Api m 1 epitope between residues 119 and 124: pIII-CVDKSKPHC (8). Control peptides: pIII-GTFDHPQ binding streptavidin (9) and pIII-CRMQMPSDC binding antihuman leptin antibodies (10). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Basophil response determined by the percentage of CD63-expressing cells in bee venom–allergic patients (patients 1-3). A, After addition of bee venom. B, After addition of nApi m 1. C, After addition of representative pIII-fused peptide mimotope. Cytokines production after 48-hour stimulation of PBMCs in bee venom–allergic patients (patients 1-3). D, With bee venom and nApi m 1. E, With representative pIII-fused peptide mimotope. The middle lines represent median. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Characterization of peptides obtained by biopanning of phage display libraries with anti–Api m 1 IgG. A, Binding of peptides displayed on phage to the immobilized target antibodies (white bars), control antiserum (gray bars), and background (black bars). B, Displacement of peptides displayed on phage from target antibodies with 1, 5, and 10 μg/mL of nApi m 1 (light gray, dark gray, and striped bars, respectively). The peptides marked in italics showed low signals and were not tested in competitive ELISA. Wild-type phage without displayed peptide served as negative control. C, Competition of peptides displayed on phage with the corresponding synthetic analogs for binding to target antibodies demonstrates that the synthetic mimotopes adopt the same conformation in solution as displayed on phage. White bars represent binding of the peptides displayed on phage to target antibodies with no synthetic peptide added. Striped, light gray, dark gray, and black bars represent the binding signal after preincubation of the same peptides displayed on phage with increasing concentration of corresponding synthetic analogs. The data are presented as the mean ± SD of 3 individual experiments. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 A, Basophil response determined by the percentage of CD63-expressing cells in bee venom–allergic patients (patients 1-3) after addition of synthetic peptide mimotopes. B, Cytokines production after 48-hour stimulation of PBMCs in bee venom–allergic patients (patients 1-3) with synthetic peptide mimotopes. The middle lines represent median. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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