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Schematic of MS1 filtering.

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Presentation on theme: "Schematic of MS1 filtering."— Presentation transcript:

1 Schematic of MS1 filtering.
Schematic of MS1 filtering.A, heat map of MS1 signal across the chromatographic gradient for the entire m/z range as acquired on a high resolution mass spectrometer. B, “zoom-in” display of an isotopic envelope for the molecular ion of a typical peptide with peaks at M, M + 1, and M + 2 selected showing changes in MS1 intensity over time. C, high resolution data allow specific filtering of molecular ions and separation of individual peaks within the isotope distribution. Skyline sums intensities within a single resolution to either side of the predicted mass to charge ratio allowing for a filter window of twice the theoretical resolution, predicted full width at half-maximum (2×FWHM). The resolution setting can be selected by the user depending on MS instrument type and preferred isotope m/z acquisition range. Birgit Schilling et al. Mol Cell Proteomics 2012;11: © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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