1. Interleukin-1β regulation of gonadotropin-releasing hormone messenger ribonucleic acid in cultured human endometrial stromal cells 
Hong-Yuan Huang, M.D., Francisco Raga, M.D., Yan Wen, M.D., Jan S Kruessel, M.D., Yung-Kuei Soong, M.D., Mary Lake Polan, M.D., Ph.D. 
Fertility and Sterility 
Volume 79, Issue 2, Pages (February 2003)
DOI: /S (02)

2. FIGURE 1
(A), A representative blot of products of PCR amplification for cultured endometrial stromal cell GnRH (S). Human luteal endometrium (ME, LE) and placenta (P) were used as positive control. ME, human midluteal endometrium; LE, human late luteal endometrium. (B), The products of nested PCR for stromal cell GnRH receptor. L = 100 bp DNA ladder.
Huang. IL-1β and GnRH in human endometrial stromal cell. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

3. FIGURE 2
A representative blot shows the standard curve obtained for GnRH mRNA by plotting the logarithmically transformed ratios of the densities of target cDNA to competitive cDNA against the log amount of initially added target cDNA in each PCR.
Huang. IL-1β and GnRH in human endometrial stromal cell. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )

4. FIGURE 3
A representative blot shows the quantitative PCR of different endometrial stromal cell samples incubated with increasing concentrations of IL-1β (0–1,000 IU/mL), demonstrating a progressive increase in the mRNA levels (A). As a control of IL-1β specificity, recombinant IL-1ra (B) and anti-IL-1β antibody (C) attenuated IL-1β-mediated up-regulation of GnRH mRNA expression in stromal cells in a dose-dependent manner. * Bars with the same symbols showed significant differences in comparison to control group (P<.05). Values are mean ± SD of measurements on three representative blots.
Huang. IL-1β and GnRH in human endometrial stromal cell. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (02) )