1. Copine RING Supplemental Figure 8 Domain abbreviations: S C D R V S CFL
+ His
PIN1
- His
Supplemental Figure 8. PIN1 interacts with RGLG2 in the
yeast two hybrid assay. The cytoplasmic domain of the
PIN-FORMED1 protein, expressed in vector pGBKT7,
interacts with the amino-terminal half of RGLG2, cloned into
vector pGAD424, in the presence of 3 mM 5-amino triazole
(-HIS), to give histidine prototrophic yeast colonies. General
assay conditions and abbreviations are as in Figure 1.
Supplemental Figure 8