1. Relative quantification of differentially expressed membrane proteins, distribution of coefficients of variation, and performance of the respiratory chain.A, relative quantification of membrane proteins showing differential accumulation levels.
Relative quantification of differentially expressed membrane proteins, distribution of coefficients of variation, and performance of the respiratory chain.A, relative quantification of membrane proteins showing differential accumulation levels. Proteins were identified by MALDI-TOF MS and PMF. If several proteins were identified in one spot, the first gene name listed corresponds to the protein with the most prominent peaks in MALDI-TOF MS spectra and the highest Mascot MOWSE protein score. Primary gene names were taken from Swiss-Prot ( Numbers refer to spot positions on the gels in Fig. 8B. A -fold change of 100 indicates a spot that was on upon membrane protein overexpression; a -fold change of 0.01 indicates that it was off. B, distribution of coefficients of variation for relative quantification of spots on 2D BN-PAGE gels. The coefficients of variation were derived from independent triplicate 2D BN-PAGE gels analyzed with PDQuest (Bio-Rad). C, performance of the respiratory chain of empty vector control cells was compared with the YidC-GFP overexpressor. Activities of succinate dehydrogenase and cytochrome oxidases were measured on inverted membrane vesicles. Oxygen consumption was measured in whole cells. Experiments were done in triplicates. Respiratory activities of control cells were set to 100.
Samuel Wagner et al. Mol Cell Proteomics 2007;6:
© 2007 The American Society for Biochemistry and Molecular Biology