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Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis  Xiulong Xu, Ph.D., Jianchi.

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Presentation on theme: "Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis  Xiulong Xu, Ph.D., Jianchi."— Presentation transcript:

1 Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis  Xiulong Xu, Ph.D., Jianchi Ding, Ph.D., Helen Ding, B.Sc., Jikun Shen, M.D., Paolo Gattuso, M.D., Richard A. Prinz, M.D., Nasir Rana, M.D., W. Paul Dmowski, M.D., Ph.D.  Fertility and Sterility  Volume 88, Issue 5, Pages (November 2007) DOI: /j.fertnstert Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Immunohistochemical analysis of heparanase-1 expression in eutopic endometrium. The sections of paraffin-embedded endometrial tissues were dewaxed and rehydrated. Heparanase-1 expression was analyzed by immunohistochemical staining with rabbit anti-heparanase-1 antiserum as described in the Materials and Methods section. Heparanase-1 expression was not present in an endometrium in the early proliferative phase (A) but was moderately expressed in an endometrium in the midsecretory phase (B) and strongly expressed in both the stromal and glandular cells in an endometrium in the late secretory phase (D). Normal rabbit serum was included as a negative control (C), showing no nonspecific staining. (E) A heparanase-1–negative ectopic endometriotic specimen; (F) a weak heparanase-1–positive ectopic endometriotic specimen; (G) a strong heparanase-1–positive specimen; (H) a heparanase-1–positive pancreatic adenocarcinoma was included as a positive control. Xu. Heparanase-1 expression in human endometriosis. Fertil Steril 2007. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions


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