1. Global visualization of antigen and epitope discovery.
Global visualization of antigen and epitope discovery.A, Concatenation of all protein profiles (similar to those in Fig. 1) ordered from left to right according to the peptide with the highest signal in each protein. The x axis shows the residue position for all concatenated proteins (full length including their antigenic and non-reactive regions), and the y axis represents the averaged, normalized and subtracted reactivity values (Chagas-specific signal). B, Enlarged region showing the antigenicity profiles of proteins that lie to the left of the selected threshold value of 3 (colored vertical line). Horizontal boxes intercalated below the concatenated profiles represent individual proteins. Blue boxes represent antigens with previously validated linear epitopes. Cyan boxes are proteins with previous serology evidence (Protein Group 4) but without known linear epitopes. Black boxes correspond to random sequences (Group 5). Green boxes represent serologically uncharacterized (‘query’) T. cruzi proteins (Groups 1, 2 and 3). C, Enrichment of different groups of proteins along the ranking. Bottom x-axis: antigenicity rank, with the most antigenic protein (according to the highest scoring peptide) at the left. Top x-axis: protein antigenicity score. In the y-axis, the cumulated fraction of proteins at each ranking position, for the different protein group.
Santiago J. Carmona et al. Mol Cell Proteomics 2015;14:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.