1. Titration of Abundance for Purified GST-Nups
7 50 mL GST-Nup
6 5 mL GST-Nup
8 1mg BSA
9 2mg BSA
10 4mg BSA
3 +IPTG
2 -IPTG
5 blank
4 MSS
1 MW
7 50 mL GST-Nup
6 5 mL GST-Nup
8 1mg BSA
9 2mg BSA
10 4mg BSA
3 +IPTG
2 -IPTG
5 blank
4 MSS
1 MW
7 50 mL GST-Nup
6 5 mL GST-Nup
8 1mg BSA
9 2mg BSA
10 4mg BSA
3 +IPTG
2 -IPTG
5 blank
4 MSS
1 MW
A GST-Nsp1h (1-172)
B GST-Nup116h ( )
C GST-Nup100h (1-640)
D GST-Nup145h (1-242)
E GST-Nup1h ( )
F GST-Nsp1 ( ) K>R
G GST-Nsp1 ( ) wild type
H GST-Nup42 (1-382)

2. Titration of Abundance for Purified GST-Nups
Group
GST-Nup
Amino Acids
Molecular Weight
(daltons) A
GST-Nsp1h (1-172)
1-172
46,653 B
GST-Nup116h ( )
83,844 C
GST-Nup100h (1-640)
1-640
93,835 D
GST-Nup145h (1-242)
1-242
53,498 E
GST-Nup1h ( )
57,092 F
GST-Nsp1 ( ) K>R
39,841 G
GST-Nsp1 ( ) wild type
39,365 H
GST-Nup42 (1-382)
1-382
67,144