1. Fig. 5 CXCL4 potentiates TLR9-mediated activation but has minimal effect on TLR7-mediated activation of pDCs purified from SSc or HDs.
CXCL4 potentiates TLR9-mediated activation but has minimal effect on TLR7-mediated activation of pDCs purified from SSc or HDs. Purified pDCs from HDs (A to C) or SSc patients (D and E) were cultured in media alone (control), either (A and D) with a CpG-C TLR9 agonist (0.1 μM) or with CpG-C and CXCL4 (10 μg/ml), or (B) with a CpG-B TLR9 agonist (0.25 μM) or with CpG-B and CXCL4, and (C and E) with a TLR7 agonist [heat-inactivated VR95 influenza virus at a MOI of 0.5 (FLU)] or with FLU and CXCL4 for 24 hours (n = 6 to 18 as indicated). IFN-α was quantified in the supernatants by ELISA. Values were normalized to 10,000 cells. All results are represented as means ± SEM, and statistical significance was evaluated using a Mann-Whitney U test. *P < 0.05, **P < 0.01, ***P <
Marie Dominique Ah Kioon et al., Sci Transl Med 2018;10:eaam8458
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