1. Exemplary validation of newly identified p53 target genes.
Exemplary validation of newly identified p53 target genes.A, qPCR analyses of MDFI, ST14, LINC01021 and miR-486 expression in SW480/pRTR-p53-VSV cells after addition of DOX for the indicated periods of time. B, qChIP validation of p53 occupancy at the MDFI, ST14, LINC01021 and miR-486 promoters in SW480/pRTR-p53-VSV after 16 h of DOX treatment and subsequent VSV-ChIP. C, p53-VSV-derived ChIP-Seq results are displayed using the UCSC genome browser. D, SW480/pRTR-LINC01021_A cell pools were subjected to impedance measurement. Twenty-four hours after seeding, DOX was added as indicated by the dotted line. E, Cell counting using trypan blue exclusion. Results in (A), (B), (D), and (E) represent the mean ± S.D. (n = 3).
Sabine Hünten et al. Mol Cell Proteomics 2015;14:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.