1. Student: Crenguța Bucătaru
University of Agronomic Sciences and Veterinary Medicine of Bucharest
Faculty of Horticulture
International Student Symposium “Hortus Academicus”
March 29, 2019, Bucharest, Romania
Genetic variability increased through intra and inter-specific hybrids in goji
Student: Crenguța Bucătaru
Assoc.Prof.Dr. Adrian Asănică
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

2. Origin Introduction Family Solanacee Gender Lycium Lycium chinense
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

3. Goji Introduction Lycium chinense Vitamins: A, C, E
Antioxidants,fiber, beta-carotene Fruits with nutraceutical effects: effect on high cholesterol, diabetes, heart disease, tuberculosis, infantile pneumonia, anemia, visual disturbances..
Goji
Lycium chinense
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

4. Materials and Methods
Location: Experimental Field of the Faculty of Horticulture, USAMV, Bucharest
Trellis system:
3 pine pillars with 3 m height
4 supporting wires at 45 cm distance between them
Planting distances: 1.2 m x 1.0 m Irrigation system: drip, flow rate 2l / hour
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

5. Goji Materials and Methods Genitors Lycium chinense Maternal parent
Patternal parent
410 C9 AS S
Lycium chinense
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

6. Materials and Methods Hybrid fruit
Choice of parents, flowers for breeding
Flower emasculation
Izolation of emasculated flowers
Breeding
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

7. Removing all petals and stamens to prevent self-pollination
Flower emasculation:
Removing all petals and stamens to prevent self-pollination
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

8. Isolation of castrated flowers:
Introduction of the branches with emasculated flower in paper bags closed at the both sides
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

9. Crossing breeding realised by touching between flowers
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

10. The seedling of hybrid seeds
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

11. Results and discussions
Genitors: 4 Lycium sp. biotypes
Maternal parent
Paternal parent
Pollinated pistils
Hybrid fruits
Seeds
number
Fruit weight average (g)
Code
410 C9 14
3/2 AS 36 6 78
0.21
5/2 S 10 1 5
0.12
6/4 16 4 34
0.4
7/4
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

12. Results and discussions
Parents
Seed
Number of rising plants
Percent
Matern
Patern
Seeds
number
Rising
410 C9
0 % AS 78 31
39,74 % S 5 34 28
82,35 %
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

13. Conclusion and reccomendation
Very good results after using the direct flower touch pollination method.
The capacity of pollen germination was between 10 % for biotype B1, 42% for biotype AS
The calendar for the start of work must be earlier, immediately after the binding for the first series of flowers
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

14. References
Asănică A., 2017 – Vertical training of berry shrubs, Total Publishing, Bucharest
Asanica A., 2015, 2016, Innovations in the fruit bushes culture, Stage I, II and III reports, USAMV Bucharest
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

15. Thank you
for your attention!