1. Induction of Metallothionein in Human Skin by Routine Exposure to Sunlight: Evidence for a Systemic Response and Enhanced Induction at Certain Body Sites 
Effie Ablett, David C. Whiteman, Glen M. Boyle, Adèle C. Green, Peter G. Parsons 
Journal of Investigative Dermatology 
Volume 120, Issue 2, Pages (February 2003)
DOI: /j x
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
MT immunohistochemistry of skin biopsies from individuals with and without recent sun exposure. Skin from the hand (a) and back (b) of an individual with no recent sun exposure showed weak immunoreactivity for MT in basal (arrow) and suprabasal (bold arrows) keratinocytes compared to that from the hand (c) and shoulder (d) of an individual with 12 h sun exposure in the 72 h prior to biopsy. The basal keratinocytes and melanocytes were more heavily stained than the suprabasal keratinocyte layers in all sections. The shoulder biopsy (d) was taken from a site covered by clothing all of the time the individual was exposed.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Quantitation of MT immunohistochemistry using image analysis. The nuclei were defined with Hoechst and the fluorescent image (a) was used to make a mask (b). The basal layer was defined manually. MT-positive cells were then identified with antibody (c) and the mask was superimposed to define the red-stained cell nuclei (d). The “density green” value of each nucleus was recorded for 100 cells in the basal or suprabasal layers (area defined previously) and plotted as a reversed histogram (e, f) where low values indicate the highest red luminosity, i.e., highest intensity of AEC staining.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Increase in epidermal MT staining with recent sun exposure of the hand. The MT immunoreactivity (SI values) of the basal (a) or suprabasal (b) layers of the epidermis of the hand is compared for individuals having no recent sun exposure, 1–11 h sun exposure, or 12–24 h exposure. SI values were calculated as shown in Fig 2. The p-values for each group indicate the significance of differences from the 0 h exposure group.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Increase in epidermal MT staining at sites other than the hand in sun-exposed individuals. The MT immunoreactivity (SI values) of epidermal basal (a) and the suprabasal (b) cells in skin from sites other than the hand. A, No recent sun exposure; B, 1–24 h exposure with the site exposed at least some of the time; C, 1–24 h exposure, site always covered by clothing. SI values were calculated as shown in Fig 2. The p-values of each group indicate the significance of differences from group A.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Induction of IL-6 expression following whole body UV exposure. Induction of IL-6 expression in leukocytes 4 h after whole-body UV exposure of two individuals (Indiv 1 and 2, respectively) to 1 MED solar-simulated UV. RT-PCR was carried out on RNA extracted from leukocytes and lymphocytes using primers specific for IL-6 and GAPDH. Both individuals showed an increased expression of IL-6 following UV exposure in purified peripheral blood leukocytes, but not lymphocytes.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions