Presentation is loading. Please wait.

Presentation is loading. Please wait.

Drug Specific Cytotoxic T-Cells in the Skin Lesions of a Patient with Toxic Epidermal Necrolysis  Amal Nassif, Armand Bensussan, Nicolas Bachot, Martine.

Published bySiska Sanjaya Modified over 5 years ago

Similar presentations

Presentation on theme: "Drug Specific Cytotoxic T-Cells in the Skin Lesions of a Patient with Toxic Epidermal Necrolysis  Amal Nassif, Armand Bensussan, Nicolas Bachot, Martine."— Presentation transcript:

1 Drug Specific Cytotoxic T-Cells in the Skin Lesions of a Patient with Toxic Epidermal Necrolysis 
Amal Nassif, Armand Bensussan, Nicolas Bachot, Martine Bagot, Laurence Boumsell, Jean-Claude Roujeau  Journal of Investigative Dermatology  Volume 118, Issue 4, Pages (April 2002) DOI: /j x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Phenotype of lymphocytes present in the blister fluid of a patient with cotrimoxazole-related TEN.(a) FACS analysis of cells present in the blister fluid and gated as lymphocytes. Most lymphocytes were CD3+, CD8+ (99%) and coexpressed CD56 (72%), CLA (83%), HLA-DR (85%). Most cells had an αβ T cell receptor (89%). (b) Cytometry analysis of the TCR Vβ repertoire. Two major Vβ chains were expressed on the blister lymphocytes (gray bars), Vβ 13.1 (56%) and Vβ14 (23%), suggesting an oligoclonal response, in contrast with normal control blood lymphocytes (black bars); however, minor populations of blister cells expressed other Vβ (not shown in the figure). Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Drug-dependent cytotoxicity of blister fluid cells on autologous lymphocytes. (a) Black bars: unstimulated blister fluid cells were lytic for autologous EVB-tranformed lymphocytes preincubated with cotrimoxazole (SMX 80 mg per liter, TMP 16 mg per liter). Blister fluid cells induced no cytotoxicity against autologous EVB-transformed lymphocytes in the absence of drug (gray bars) and against unrelated allogeneic cells preincubated with cotrimoxazole (white bars). (b) Preincubation of target cells with sulfamethoxazole-SMX alone, 80 mg per liter (white bar) or with the nitroso metabolite 20 mg per liter (dotted bar) resulted in a lysis by blister fluid cells similar to that observed with cotrimoxazole, i.e., SMX 80 mg per liter plus TMP 16 mg per liter (gray bar). The hydroxylamine metabolite of sulfamethoxazole (black bar) had no significant effect as compared with the medium alone (dark gray bar) Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 MHC class I restriction of drug-dependent cytotoxicity.(a) Anti-class I monoclonal antibody W6-32, but not an anti-class II, abolished drug-dependent lysis of autologous lymphocytes by blister fluid cells. Grey bars, lysis of target cells without drug; black bars, lysis of target cells in the presence of cotrimoxazole (SMX 80 mg per liter plus TMP 16 mg per liter); white bars, lysis of target cells in the presence of both cotrimoxazole and anti-class I monoclonal antibody. Dark gray bars, lysis of target cells in the presence of both cotrimoxazole and anti-class II monoclonal antibody. (b) Blister fluid cells were tested, at an effector/target ratio of 40/1, in the presence of cotrimoxazole on EBV-transformed lymphocytes from two allogeneic controls sharing with the patient a variety of serologically determined HLA class I molecules and on a mutant cell line C1R, expressing only CW4 by serologic determination. Lysis segregated with HLA-Cw4. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Mediators of cytotoxicity.(a) Blister cells preincubated with IL-2 (100 UI per ml for 24 h) exhibited an increased specific drug-dependent cytotoxicity (black bar), but no LAK cytotoxicity on target cells in the absence of drug (gray bar). This drug-dependent lysis was totally abolished by preincubation of target cells with EGTA (dark gray bar) but not by preincubation with monoclonal antibodies anti-CD95-Fas (white bar) or anti-TRAIL R1 and R2 (TRAIL) (dotted bar).(b) When effector cells were preincubated with Brefeldin A (white bar) the drug-dependent lysis (black bar) was unaffected. In contrast it was abolished by CMA (dark gray bar). (c) Although not implicated in the lysis, CD95-Fas and TRAIL receptor 2 were expressed on target lymphocytes as shown by FACS analysis. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Drug Specific Cytotoxic T-Cells in the Skin Lesions of a Patient with Toxic Epidermal Necrolysis  Amal Nassif, Armand Bensussan, Nicolas Bachot, Martine."

Similar presentations

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides  Tilmann Kallinich, J. Marcus Muche,

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides  Tilmann Kallinich, J. Marcus Muche,
A Promiscuous Survivin-Derived T-Cell Epitope Restricted to the HLA-A3 Super-Type Alleles  Niels Junker, Shamaila Munir, Pia Kvistborg, Per thor Straten,

A Promiscuous Survivin-Derived T-Cell Epitope Restricted to the HLA-A3 Super-Type Alleles  Niels Junker, Shamaila Munir, Pia Kvistborg, Per thor Straten,
CD158k Is a Reliable Marker for Diagnosis of Sézary Syndrome and Reveals an Unprecedented Heterogeneity of Circulating Malignant Cells  Hélène Moins-Teisserenc,

CD158k Is a Reliable Marker for Diagnosis of Sézary Syndrome and Reveals an Unprecedented Heterogeneity of Circulating Malignant Cells  Hélène Moins-Teisserenc,
UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,

UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,
Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia- Influenced Cell Interaction  Eva Buentke, Lena C. Heffler, Annika.

Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia- Influenced Cell Interaction  Eva Buentke, Lena C. Heffler, Annika.
MART-1- and gp100-Expressing and -Non-Expressing Melanoma Cells Are Equally Proliferative in Tumors and Clonogenic In Vitro  Mariana Aris, Mariana Rodríguez.

MART-1- and gp100-Expressing and -Non-Expressing Melanoma Cells Are Equally Proliferative in Tumors and Clonogenic In Vitro  Mariana Aris, Mariana Rodríguez.
Systemic Photodynamic Therapy with Aminolevulinic Acid Induces Apoptosis in Lesional T Lymphocytes of Psoriatic Plaques  Robert Bissonnette, Dr., Jean-François.

Systemic Photodynamic Therapy with Aminolevulinic Acid Induces Apoptosis in Lesional T Lymphocytes of Psoriatic Plaques  Robert Bissonnette, Dr., Jean-François.
Specific Lysis of Melanoma Cells by Receptor Grafted T Cells is Enhanced by Anti- Idiotypic Monoclonal Antibodies Directed to the scFv Domain of the Receptor 

Specific Lysis of Melanoma Cells by Receptor Grafted T Cells is Enhanced by Anti- Idiotypic Monoclonal Antibodies Directed to the scFv Domain of the Receptor 
HLA-A*0201+ Plasmacytoid Dendritic Cells Provide a Cell-Based Immunotherapy for Melanoma Patients  Caroline Aspord, Marie-Therese Leccia, Dimitri Salameire,

HLA-A*0201+ Plasmacytoid Dendritic Cells Provide a Cell-Based Immunotherapy for Melanoma Patients  Caroline Aspord, Marie-Therese Leccia, Dimitri Salameire,
Methods to Improve Adoptive T-Cell Therapy for Melanoma: IFN-γ Enhances Anticancer Responses of Cell Products for Infusion  Marco Donia, Morten Hansen,

Methods to Improve Adoptive T-Cell Therapy for Melanoma: IFN-γ Enhances Anticancer Responses of Cell Products for Infusion  Marco Donia, Morten Hansen,
Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen 

Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen 
Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.

Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.
The IL-17A-Producing CD8+ T-Cell Population in Psoriatic Lesional Skin Comprises Mucosa-Associated Invariant T Cells and Conventional T Cells  Marcel.

The IL-17A-Producing CD8+ T-Cell Population in Psoriatic Lesional Skin Comprises Mucosa-Associated Invariant T Cells and Conventional T Cells  Marcel.
IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.
KIR3DL2/CpG ODN Interaction Mediates Sézary Syndrome Malignant T Cell Apoptosis  Bouchra Ghazi, Nicolas Thonnart, Martine Bagot, Armand Bensussan, Anne.

KIR3DL2/CpG ODN Interaction Mediates Sézary Syndrome Malignant T Cell Apoptosis  Bouchra Ghazi, Nicolas Thonnart, Martine Bagot, Armand Bensussan, Anne.
T-Cell Receptor Repertoire and Cytokine Pattern in Granuloma Annulare: Defining a Particular Type of Cutaneous Granulomatous Inflammation  Martin Mempel,

T-Cell Receptor Repertoire and Cytokine Pattern in Granuloma Annulare: Defining a Particular Type of Cutaneous Granulomatous Inflammation  Martin Mempel,
FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.
Donor T Cells Administered Over HLA Class II Barriers Mediate Antitumor Immunity without Broad Off-Target Toxicity in a NOD/Scid Mouse Model of Acute.

Donor T Cells Administered Over HLA Class II Barriers Mediate Antitumor Immunity without Broad Off-Target Toxicity in a NOD/Scid Mouse Model of Acute.
Oxypurinol-Specific T Cells Possess Preferential TCR Clonotypes and Express Granulysin in Allopurinol-Induced Severe Cutaneous Adverse Reactions  Wen-Hung.

Oxypurinol-Specific T Cells Possess Preferential TCR Clonotypes and Express Granulysin in Allopurinol-Induced Severe Cutaneous Adverse Reactions  Wen-Hung.
A Reducing Microenvironment Leads to the Generation of FcεRIhigh Inflammatory Dendritic Epidermal Cells (IDEC)  Natalija Novak, MD, Stefan Kraft, Jörg.

A Reducing Microenvironment Leads to the Generation of FcεRIhigh Inflammatory Dendritic Epidermal Cells (IDEC)  Natalija Novak, MD, Stefan Kraft, Jörg.

Similar presentations

Ads by Google