1. Obtaining cherry varieties by conducted hybridation in Istrița
University of Agronomic Sciences and Veterinary Medicine of Bucharest
Faculty of Horticulture
International Student Symposium “Hortus Academicus”
March 29, 2019, Bucharest, Romania
Obtaining cherry varieties by conducted hybridation in Istrița
Student: Ionuț Albei
Prof. Asoc. Dr. Adrian Asanică
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

2. Introduction
Cherry is a fruit species of great economic importance because of the nutritional property, technological and commercial attributes of the fruits and the biological properties of the trees to capitalize on a wide variety of pedoclimatic conditions in the plain, hilly or even submontane areas.
Origin
Order: Rosales
Prunus avium
Family: Rosaceae
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

3. Materials and methods Celeste ♂ Kordia ♂ Firm Red ♂ Early Red ♂
Location: Istriţa Nursery, Buzău County
Genitors
New Star ♀
Celeste ♂
Kordia ♂
Firm Red ♂
Early Red ♂
Giant Red ♂
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

4. Materials and methods Conducted hybridation
The choice of genitors, buds or flowers for pollination
Emasculation the flowers on the chosen branches
Izolation of emasculated flowers
Harvesting and preserving pollen until use
Pollination
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

5. Emasculation the flowers on the chosen branches
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

6. Izolation of emasculated flowers
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

7. Harvesting and preserving pollen until use
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

8. Pollination
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

9. Harvesting hybrids and extracting seeds
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

10. Stratification of the seeds
Keep the seeds in the refrigerator for 60 days at 4 °C
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

11. Scoaterea sâmburilor de la stratificat și semănarea acestora
Înainte de semănare se verifica viabilitatea sâmburilor și se seamănă doar cei viabili.
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

12. Results and discussions
Genitors: 6 Prunus avium biotypes
Hybrid combination 
Number of castrated and pollinated flower
Hybrid fruits
 Number of planting seeds
Nr. %
♀New Star x ♂Celeste
630
147
23,33
 50
♀New Star x ♂Kordia
400 56 14
 36
♀New Star x ♂Firm Red
977
240
24,56
96 
♀New Star x ♂Early Red
869
304
34,98
 96
♀New Star x ♂Giant Red
798
294
36,84
 120
Total
3674
1041
26,74
 398
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

13. Rezultate si discuții Hybrid combination Hybrid fruits
Hybrid fruits 
Number of planting seeds
Number of rising plants
Rising
♀New Star x ♂Celeste
147
 50 2 4%
♀New Star x ♂Kordia 56
 36 0%
♀New Star x ♂Firm Red
240
96 
♀New Star x ♂Early Red
304
 96
♀New Star x ♂Giant Red
294
 120 3 6%
International Student Symposium "Hortus Academicus“ March 29, Bucharest, Romania

14. Conclusions and recommendations
Climate conditions had a very important role in successfully conducting hybridation operations, with unfavorable climatic conditions adversely affecting the number of hybrids and hybrids produced.
New Star had good compatibility with most varieties with which it was crossed, especially with the Early Red and Giant Red varieties, with even more than 30% hybrids in 2018.
A very low sampling rate has been observed due to stratification over a longer period, we recommend that the stratification period be maximum 120 days.
In 2018 a mean percentage of 26.74% of hybrid fruit was obtained
The Early Red and Giant Red varieties gave the best results as paternal genres (♂).
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania

15. Thank you
for your attention!