1. Target identification of ampicillin and ciprofloxacin
Target identification of ampicillin and ciprofloxacin 2D‐TPP protocol overview. After treatment with different concentrations of antibiotics, the cells were prepared in a similar manner to what is described in Fig 1A. For each protein and temperature, the signal intensity was normalized to the vehicle control.Heatmaps for targets of ampicillin in living cells, with coloring according to panel (A). *FDR controlled at 1% using a bootstrapped permutation approach.Number of stabilized or destabilized proteins in lysate and living cells after treatment with ampicillin.Example of top gene ontology terms enriched in proteins affected in living cells after treatment with ampicillin.Heatmaps for targets of ciprofloxacin in living cells, with coloring according to what is described in panel (A). *FDR controlled at 1% using a bootstrapped permutation approach.Schematic representation of SOS response. LexA binds to promoters of SOS response genes and represses their transcription. Treatment with ciprofloxacin induces single‐ and double‐stranded DNA breaks that recruit RecA to DNA, causing auto‐cleavage of LexA and expression of SOS response genes (e.g., yebG; Andersson & Hughes, 2014).
André Mateus et al. Mol Syst Biol 2018;14:e8242
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