1. Optimal Reference Gene Selection for Expression Studies in Human Reticulocytes 
Anu Aggarwal, Manu Jamwal, Ganesh K. Viswanathan, Prashant Sharma, ManUpdesh S. Sachdeva, Deepak Bansal, Pankaj Malhotra, Reena Das 
The Journal of Molecular Diagnostics 
Volume 20, Issue 3, Pages (May 2018)
DOI: /j.jmoldx
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Box-plot graph depicting expression levels [threshold cycle (Ct) values] of the seven candidate reference genes across the entire cohort of experimental samples. Ct value shows the median values as lines across the box. Lower and upper boxes indicate 25% to 75%. Whiskers represent the maximum and minimum values. n = 45 experimental samples.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Scatter plot of fold-change for six of the reference genes derived from real-time quantitative PCR analysis across hereditary spherocytosis (HS) and β-thalassemia intermedia (βTI) samples. Each dot represents data obtained from a single patient, and the bar represents the median fold-change. HPRT was excluded from the data because of large variation.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions