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Moderate hypothermia during cardiopulmonary bypass increases intramyocardial synthesis of heat shock protein 72  Ma Qing, MD, Jaime F. Vazquez-Jimenez,

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Presentation on theme: "Moderate hypothermia during cardiopulmonary bypass increases intramyocardial synthesis of heat shock protein 72  Ma Qing, MD, Jaime F. Vazquez-Jimenez,"— Presentation transcript:

1 Moderate hypothermia during cardiopulmonary bypass increases intramyocardial synthesis of heat shock protein 72  Ma Qing, MD, Jaime F. Vazquez-Jimenez, MD, Kathrin Schumacher, MD, Ranjit S. Bhardwaj, PhD, Bernd Klosterhalfen, MD, Ralf Minkenberg, Bruno J. Messmer, MD, Götz von Bernuth, MD, Marie-Christine Seghaye, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 124, Issue 4, Pages (October 2002) DOI: /mtc Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

2 Fig. 1 A, Expression of mRNA encoding HSP-72 in myocardium before CPB (1), before aortic crossclamping (2), before removal of aortic clamp (3), and 6 hours after CPB (4) in pigs operated on under moderate hypothermia (temperature 28°C, circles) and under normothermia (temperature 37°C, squares). Results are shown as ratio of mRNA encoding HSP-72 to that encoding β-actin detected by RT-PCR. Data points represent mean value; error bars represent SEM. Double asterisk indicates difference versus prebypass value in hypothermia group (P =.042). Asterisk indicates P <.001 between groups; dagger indicates P =.002; double dagger indicates P =.007. B, Effect of core temperature on myocardial expression of mRNA encoding HSP-72 detected by RT-PCR. Results are representative for 8 independent experiments in each group (moderate hypothermia 28°C, normothermia 37°C). M, Molecular weight markers; bp, base pairs. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

3 Fig. 2 A. Synthesis of HSP-72 measured by Western blot in myocardium before CPB (1), before aortic crossclamping (2), before removal of aortic clamp (3), and 6 hours after CPB (4) in pigs operated on under moderate hypothermia (temperature 28°C, circles) and under normothermia (temperature 37°C, squares). Band intensities for HSP-72 were normalized for band intensities of β-actin. Data points represent mean value; error bars represent SEM. Double asterisk indicates difference versus prebypass value in hypothermia group (P =.068). Asterisk indicates P =.001 between groups; dagger indicates P =.085; double dagger indicates P =.058. B, Effect of core temperature on myocardial expression of HSP-72 detected by Western blot. Results are representative for 8 independent experiments in each group (moderate hypothermia 28°C, normothermia 37°C). M, Molecular weight markers. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

4 Fig. 3 Example of immunohistochemical study (original magnification 1000×) of right ventricular myocardium taken 6 hours after CPB in representative animal operated on under hypothermia showing the presence of HSP-72 (red staining) within myocardial cells (My, arrows), endothelial cells (E, arrows), and resident macrophages (M, arrows). C, Control probe taken before CPB. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

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