Presentation is loading. Please wait.

Presentation is loading. Please wait.

Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia/reperfusion  Aditya K. Kaza, MD, Isaac Wamala, MD, Ingeborg.

Published byAnn Aas Modified over 6 years ago

Similar presentations

Presentation on theme: "Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia/reperfusion  Aditya K. Kaza, MD, Isaac Wamala, MD, Ingeborg."— Presentation transcript:

1 Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia/reperfusion  Aditya K. Kaza, MD, Isaac Wamala, MD, Ingeborg Friehs, MD, Joseph D. Kuebler, MD, Rahul H. Rathod, MD, Ignacio Berra, MD, Maria Ericsson, BS, Rouan Yao, BS, Jerusha K. Thedsanamoorthy, BS, David Zurakowski, PhD, Sidney Levitsky, MD, Pedro J. del Nido, MD, Douglas B. Cowan, PhD, James D. McCully, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 153, Issue 4, Pages (April 2017) DOI: /j.jtcvs Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 A, Experimental protocol. Injection sites in the area at risk are indicated. B, Representative electron micrographs of isolated pig skeletal muscle mitochondria. The isolated mitochondria were free from cellular contamination and were electron dense and had preserved morphology and shape. (Scale bars: 1 μm.) RI, Regional ischemia; ECG, echocardiography. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 Body weight (A) and heart weight (B). There was no significant difference in body weight between groups either at the time of initial or terminal surgery. The mean change in body weight over 4 weeks (δ) is shown in (A). There was no significant difference in left ventricular weight at the time of initial or terminal surgery (B). LV, Left ventricular. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 Creatine kinase-MB isoenzyme (CK-MB) (A) and cardiac troponin I (cTnI) (B) at day 1 and day 3 after surgery in the vehicle-only and mitochondria groups. Both CK-MB and cTnI were significantly decreased (P < .001) in mitochondria hearts compared with vehicle hearts at day 3. There was no significant difference between the 2 groups at day 1. Results are shown for n = 6 in each group. Significant differences compared with vehicle are shown. ∗Significant differences compared with vehicle at P = .006. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 A and B, Area at risk (AAR) (A) and infarct size (%AAR) (B) for vehicle and mitochondria. Results are shown as the mean ± standard deviation for n = 6 in each group. Significant differences compared with vehicle are shown. C and D, Histochemical and electron microscopy analysis of myocardial tissue at 4 weeks of recovery. Representative Masson's trichrome-stained (C) and transmission electron (D) micrographs from vehicle and mitochondria hearts are shown. Longitudinal and transverse interfibrillar separation was observed in vehicle hearts but not in mitochondria hearts (C), but there was no difference in collagen between the 2 groups (C). Electron microscopy analysis (D) shows mitochondrial damage and contraction bands in vehicle hearts that are not present in mitochondria hearts. (Scale bars: 500 μm.) LV, Left ventricular. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

6 Figure 5 A, Electrophysiological data. Representative electrocardiograms at baseline (before surgery), ischemia (following 10 minutes of regional ischemia), and reperfusion (following 120 minutes of reperfusion). B, Echocardiography data. A, Recommended 17-segment model for the left ventricle.14 B, Global and regional circumferential strain analysis from vehicle and mitochondria hearts. Strain was measured in the midventricular short-axis plane (segments 7-12). More negative strain values indicate a more favorable strain. Values are shown as mean ± standard deviation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

7 Figure 6 Mitochondrial uptake at 4 weeks after injection. A and B, T2*-weighted magnetic resonance imaging of mitochondrial uptake in pig heart at 4 weeks after injection. Iron oxide–labeled mitochondria appear as dark regions in the heart (arrows). Coronal (left) and transverse slices (right) are depicted from 2 different regions of the same heart. C, Histological sections from the dark regions were stained for iron (left) and human mitochondria (right). Our results show that mitochondria are present for at least 4 weeks after injection in the pig heart. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

8 Autologous mitochondrial transplantation decreases infarct size and preserves structure.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

9 The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia/reperfusion  Aditya K. Kaza, MD, Isaac Wamala, MD, Ingeborg."

Similar presentations

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,
Manuel J. Antunes, MD, PhD, DSc 

Manuel J. Antunes, MD, PhD, DSc 
Effects of myocardial ischemia on the release of cardiac troponin I in isolated rat hearts  Sidney Chocron, MDa, Kifah Alwan, MDa, Gerard Toubin, MDb,

Effects of myocardial ischemia on the release of cardiac troponin I in isolated rat hearts  Sidney Chocron, MDa, Kifah Alwan, MDa, Gerard Toubin, MDb,
Simvastatin attenuates cardiac isograft ischemia-reperfusion injury by down-regulating CC chemokine receptor-2 expression  Rong Yin, MD, Jiaquan Zhu,

Simvastatin attenuates cardiac isograft ischemia-reperfusion injury by down-regulating CC chemokine receptor-2 expression  Rong Yin, MD, Jiaquan Zhu,
Multigene adenoviral therapy for the attenuation of ischemia-reperfusion injury after preservation for cardiac transplantation  Haitham J. Abunasra, FRCS,

Multigene adenoviral therapy for the attenuation of ischemia-reperfusion injury after preservation for cardiac transplantation  Haitham J. Abunasra, FRCS,
Hemodynamics and myocardial blood flow patterns after placement of a cardiac passive restraint device in a model of dilated cardiomyopathy  Jennifer A.

Hemodynamics and myocardial blood flow patterns after placement of a cardiac passive restraint device in a model of dilated cardiomyopathy  Jennifer A.
Electron transport chain dysfunction in neonatal pressure-overload hypertrophy precedes cardiomyocyte apoptosis independent of oxidative stress  Eric.

Electron transport chain dysfunction in neonatal pressure-overload hypertrophy precedes cardiomyocyte apoptosis independent of oxidative stress  Eric.
Fixed versus dynamic subaortic stenosis: Hemodynamics and resulting differences in Doppler echocardiography and aortic pressure contour  Joon Hwa Hong,

Fixed versus dynamic subaortic stenosis: Hemodynamics and resulting differences in Doppler echocardiography and aortic pressure contour  Joon Hwa Hong,
Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.
A novel peroxynitrite decomposer catalyst (FP-15) reduces myocardial infarct size in an in vivo peroxynitrite decomposer and acute ischemia-reperfusion.

A novel peroxynitrite decomposer catalyst (FP-15) reduces myocardial infarct size in an in vivo peroxynitrite decomposer and acute ischemia-reperfusion.
Protective effects of protein kinase C during myocardial ischemia require activation of phosphatidyl-inositol specific phospholipase C  Mamoru Munakata,

Protective effects of protein kinase C during myocardial ischemia require activation of phosphatidyl-inositol specific phospholipase C  Mamoru Munakata,
Prolonged donor heart preservation with pinacidil: The role of mitochondria and the mitochondrial adenosine triphosphate–sensitive potassium channel 

Prolonged donor heart preservation with pinacidil: The role of mitochondria and the mitochondrial adenosine triphosphate–sensitive potassium channel 
Is hyperglycemia bad for the heart during acute ischemia?

Is hyperglycemia bad for the heart during acute ischemia?
Per Steinar Halvorsen, MD, Espen W

Per Steinar Halvorsen, MD, Espen W
Bone marrow–derived mononuclear cell transplantation improves myocardial recovery by enhancing cellular recruitment and differentiation at the infarction.

Bone marrow–derived mononuclear cell transplantation improves myocardial recovery by enhancing cellular recruitment and differentiation at the infarction.
Transgenic model of cardiac rhabdomyosarcoma formation

Transgenic model of cardiac rhabdomyosarcoma formation
Acute hyperglycemia enhances oxidative stress and exacerbates myocardial infarction by activating nicotinamide adenine dinucleotide phosphate oxidase.

Acute hyperglycemia enhances oxidative stress and exacerbates myocardial infarction by activating nicotinamide adenine dinucleotide phosphate oxidase.
Diazoxide amelioration of myocardial injury and mitochondrial damage during cardiac surgery  James D McCully, PhD, Hidetaka Wakiyama, MD, Douglas B Cowan,

Diazoxide amelioration of myocardial injury and mitochondrial damage during cardiac surgery  James D McCully, PhD, Hidetaka Wakiyama, MD, Douglas B Cowan,
Ethyl pyruvate preserves cardiac function and attenuates oxidative injury after prolonged myocardial ischemia  Y.Joseph Woo, MD, Matthew D Taylor, BS,

Ethyl pyruvate preserves cardiac function and attenuates oxidative injury after prolonged myocardial ischemia  Y.Joseph Woo, MD, Matthew D Taylor, BS,
Using contracting band to improve right ventricle ejection fraction for patients with repaired tetralogy of Fallot: A modeling study using patient-specific.

Using contracting band to improve right ventricle ejection fraction for patients with repaired tetralogy of Fallot: A modeling study using patient-specific.

Similar presentations

Ads by Google