1. ProteinChip⁎ technology is a useful method in the pathogenesis and diagnosis of endometriosis: a preliminary study 
Cleophas M. Kyama, M.Sc., Davy T’Jampens, Ph.D., Attila Mihalyi, Ph.D., Peter Simsa, M.Sc., Sophie Debrock, Ph.D., Etienne Waelkens, M.D., Ph.D., Bart Landuyt, M.Sc., Christel Meuleman, M.D., Vilmos Fulop, M.D., Jason M. Mwenda, Ph.D., Thomas M. D’Hooghe, M.D., Ph.D. 
Fertility and Sterility 
Volume 86, Issue 1, Pages (July 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
(A) Up-regulation of 22- to 23-kDa cluster in endometriotic lesion biopsy lysates. A higher expression level of 22- to 23-kDa peptides can be observed in the endometriotic lesions (EL) from patient 1 and 2 after SELDI-TOF-MS spectrum profiling of proteins retained on the CM10 surface with pH 9.0 binding condition, SPA as energy-absorbing molecules. X-axis: m/z (Da), zoom in on the 20- to 24-kDa region of the peptide spectrum. Y-axis: relative ratio (%) to the highest signal in the spectrum. PB = peritoneal biopsy). (B) Confirmation of transgelin in endometriotic lesion lysates of patients 1 (EL1) and 2 (EL2).
Kyama. SELDI-TOF MS in endometriosis reaction. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Identification of 22- to 23-kDa proteins as transgelin. (A) 4–12% NuPage gel. Lane 1, Mark12 molecular weight marker; lane 2, normal peritoneal biopsy lysate; lane 3, endometriotic lesion biopsy lysate; lane 4, blank; and lane 5, Mark12 molecular weight marker. Band I was excised from gel, with one part subjected to 2-hour passive elution of intact protein present in this gel and the other part subjected to 2-hour in-gel tryptic digest. (B) Coverage map of observed in-gel tryptic peptides matching with 57% of the complete transgelin amino acid sequence, as listed in the National Center for Biotechnology Information-nonredundant database. (C) Surface enhanced laser desorption/ionization–TOF-MS spectrum visualizing passive elution product of protein band (16% Tricine gel), yielding mainly a 22.9-kDa peak. (D) Collision-induced dissociation spectrum of the 1, Da peptide resulting in a series of y ions assisting in confirmatory identification of this peptide as the K (21–29) R peptide (KYDEELEER) of transgelin, a smooth muscle actin–binding protein.
Kyama. SELDI-TOF MS in endometriosis reaction. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions