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J. Trent Magruder, MD, Todd C

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1 Selective Localization of a Novel Dendrimer Nanoparticle in Myocardial Ischemia- Reperfusion Injury 
J. Trent Magruder, MD, Todd C. Crawford, MD, Yi-An Lin, PhD, Fan Zhang, PhD, Joshua C. Grimm, MD, Rangaramanujam M. Kannan, PhD, Sujatha Kannan, MD, Christopher M. Sciortino, MD, PhD  The Annals of Thoracic Surgery  Volume 104, Issue 3, Pages (September 2017) DOI: /j.athoracsur Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Timeline of experimental plan. (G6-Cy5 = generation 6 dendrimer conjugated to cyanine-5 fluorescent dye; IV = intravenously; LAD = left anterior descending coronary artery.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 Dendrimer levels in serum at 1 hour, 3 hours, 24 hours, and 48 hours postoperatively for myocardial ischemia (MI) (red) and control animals (blue). Black bars denote standard errors. Comparing MI versus control animals, p = 0.37 by repeated measures analysis of variance. (G6-Cy5 = generation 6 dendrimer conjugated to cyanine-5 fluorescent dye.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Dendrimer levels in urine at 3-hour, 24-hour, and 48-hour postoperative sacrifice time points for myocardial ischemia (MI) (red) and control animals (blue). Black bars denote standard errors. Comparing MI versus control animals, p = 0.30 by repeated measures analysis of variance. Asterisk denotes p < 0.05 on post hoc test. (G6-Cy5 = generation 6 dendrimer conjugated to cyanine-5 fluorescent dye; MI = myocardial ischemia animals.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Confocal microscopy demonstrating qualitatively increased G6-Cy5 uptake (red fluorescence) in (A) the anterior wall as compared to (B) the posterior myocardial wall at 48 hours. Blue fluorescence denotes 4`,6-diamidino-2-phenylindole fluorescent stain (DAPI), which stains for DNA. (G6-Cy5 = generation 6 dendrimer conjugated to cyanine-5 fluorescent dye.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Confocal microscopy demonstrating only modest colocalization of G6-Cy5 with macrophages (arrows) at both 24 and 48 hours post–myocardial ischemia. (DAPI = 4`,6-diamidino-2-phenylindole fluorescent stain (for DNA); G6-Cy5 = generation 6 dendrimer conjugated to cyanine-5 fluorescent dye; HTI = ischemic heart tissue.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2017 The Society of Thoracic Surgeons Terms and Conditions

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