1. Gap2 is required for growth on phenylalanine as the sole source of nitrogen.
Gap2 is required for growth on phenylalanine as the sole source of nitrogen. Cells from an overnight grown culture on YPD plates were resuspended and diluted to an OD600 of 1 for drop tests or to an OD600 of for liquid growth assays. (A) Three microliters of 10-fold dilutions were spotted on YNB medium with phenylalanine (300 µg/ml) or ammonium sulfate (5 mg/ml) as the sole source of nitrogen. Pictures were taken after 2 days. (B) For the liquid growth assays, YNB medium (supplemented with 300 µg/ml of phenylalanine or ammonium sulfate [5 mg/ml] as the sole source of nitrogen) was used, and 100-µl aliquots were incubated at 30°C in 96-well microtiter plates in an absorbance microplate reader. The optical density in each well was measured over a period of 24 h. All strains that had a deletion in GAP2 were unable to grow in medium containing phenylalanine as the sole source of nitrogen. The inset in the lower left panel shows the absence of growth (OD from 0.07 to 0.08 over a period of 24 h).
Lucie Kraidlova et al. mSphere 2016; doi: /mSphere