1. Partial sequencing of the VP2 capsid gene for direct enterovirus genotyping in clinical specimens 
W. Ibrahim, N. Boukhadra, D. Nasri-Zoghlami, P. Berthelot, S. Omar, T. Bourlet, B. Pozzetto, S. Pillet 
Clinical Microbiology and Infection 
Volume 20, Issue 9, Pages O558-O565 (September 2014)
DOI: /
Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

2. FIG. 1
Set-up of the CODEHOP VP2 typing method. (a) Design of the NS20 primer (the same strategy was used for NS30). After alignment of the sequences of EV, including the newly described ones, the conserved blocks were determined according to The amino acid block corresponding to the region previously used for the AM21 and AM22 primers was selected. The targeted sequence of the block is shown in a red box. (b) Design of the NS20 primer. The most frequent amino acid was selected in each position of the block, and the nucleotide sequence was designed with a 5’ consensus clamp and a 3’ degenerate core according to the CODEHOP strategy. (c) Electrophoregrams of amplification products of a variety of enterovirus strains belonging to different species after use of either the initial version (left-side panels) of the VP2 typing method described in [17], or the CODEHOP version of this test (right-side panels). The strains used are those listed in Table 1 at the same multiplicity of infection.
Clinical Microbiology and Infection  , O558-O565DOI: ( / )
Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

3. FIG. 2
Box plots comparing the Ct values obtained with (a) the GeneXpert test and (b) the R-gene test targeting the 5′NC region in two groups of samples according to their typeability by use of the VP2 method. The median Ct value obtained was lower for typeable samples than for untypeable ones (32.20 vs , p <0.05 for the GeneXpert test, and vs , p <0.001 for the R-gene test, by use of the Mann-Whitney-Wilcoxon test).
Clinical Microbiology and Infection  , O558-O565DOI: ( / )
Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions