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In vitro effects of peroxisome proliferator–activated receptor-γ ligands on gene expression in lipopolysaccharide-induced endometrial and endometriotic.

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Presentation on theme: "In vitro effects of peroxisome proliferator–activated receptor-γ ligands on gene expression in lipopolysaccharide-induced endometrial and endometriotic."— Presentation transcript:

1 In vitro effects of peroxisome proliferator–activated receptor-γ ligands on gene expression in lipopolysaccharide-induced endometrial and endometriotic stromal cells  Indu Sharma, Ph.D., Veena Dhawan, Ph.D., Subhash Chand Saha, M.D., Lakhbir Kaur Dhaliwal, M.D.  Fertility and Sterility  Volume 95, Issue 2, Pages e5 (February 2011) DOI: /j.fertnstert Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2 Time- and dose-dependent effects of rosiglitazone and 15d-PGJ2 on LPS-induced mRNA expression of PPAR-γ, LXR-α, and COX-2 genes in ESCs of group 1 (n = 15) and 2 (n = 10) subjects. Solid line = rosiglitazone; broken line = 15d-PGJ2; a = rosiglitazone/15d-PGJ2-treated versus LPS-induced mRNA expression; b = 0.1 μM versus 1.0 μM; c = 1.0 μM versus 10 μM; d = 0.1 μM versus 10 μM; e = 3 hours versus 6 hours; f = 6 hours versus 9 hours; g = 9 hours versus 12 hours; h = rosiglitazone treated versus 15d-PGJ2 treated; ∗P<.05; $P<.01; #P<.001. Fertility and Sterility  , e5DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3 Time- and dose-dependent effects of rosiglitazone and 15d-PGJ2 on LPS-induced protein expression of PPAR-γ, LXR-α, and COX-2 genes in ESCs of group 1 (n = 15) and 2 (n = 10) subjects. Solid Complete line = rosiglitazone; broken line = 15d-PGJ2; a = rosiglitazone/15d-PGJ2-treated versus LPS-induced mRNA expression; b = 0.1 μM versus 1.0 μM; c = 1.0 μM versus 10 μM; d = 0.1 μM versus 10 μM; e = 3 hours versus 6 hours; f = 6 hours versus 9 hours; g = 9 hours versus 12 hours; h = rosiglitazone treated versus 15d-PGJ2 treated; ∗P<.05; $P<.01; #P<.001. Fertility and Sterility  , e5DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4 Time- and dose-dependent effects of rosiglitazone and 15d-PGJ2 on LPS-induced mRNA expression of VEGF, RAGE, and EN-RAGE genes in ESCs of group 1 (n = 15) and 2 (n = 10) subjects. Solid Complete line = rosiglitazone; broken line = 15d-PGJ2; a = rosiglitazone/15d-PGJ2-treated versus LPS-induced mRNA expression; b = 0.1 μM versus 1.0 μM; c = 1.0 μM versus 10 μM; d = 0.1 μM versus 10 μM; e = 3 hours versus 6 hours; f = 6 hours versus 9 hours; g = 9 hours versus 12 hours; h = rosiglitazone treated versus 15d-PGJ2 treated; ∗P<.05; $P<.01; #P<.001. Fertility and Sterility  , e5DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5 Time- and dose-dependent effects of rosiglitazone and 15d-PGJ2 on LPS-induced protein expression of VEGF, RAGE, and EN-RAGE genes in ESCs of group 1 (n = 15) and 2 (n = 10) subjects. Solid Complete line = rosiglitazone; broken line = 15d-PGJ2; a = rosiglitazone/15d-PGJ2-treated versus LPS-induced mRNA expression; b = 0.1 μM versus 1.0 μM; c = 1.0 μM versus 10 μM; d = 0.1 μM versus 10 μM; e = 3 hours versus 6 hours; f = 6 hours versus 9 hours; g = 9 hours versus 12 hours; h = rosiglitazone-treated versus 15d-PGJ2-treated; ∗P<.05; $P<.01; #P<.001. Fertility and Sterility  , e5DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions


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