1. The Role of Neutrophilic Inflammation, Angiotropism, and Pericytic Mimicry in Melanoma Progression and Metastasis 
Jennifer Landsberg, Thomas Tüting, Raymond L. Barnhill, Claire Lugassy 
Journal of Investigative Dermatology 
Volume 136, Issue 2, Pages (February 2016)
DOI: /j.jid
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2. Figure 1
Angiotropism and pericytic mimicry of melanoma cells. (a) Angiotropism in a human primary melanoma. V = vessel. Scale bar = 50 μm. Reprinted with permission from Barnhill and Lugassy (2004). (b) Angiotropism in a metastasis (immunostaining for MelanA and CD31). Scale bar = 50 μm. (c) Angiotropism of the neural crest stem cell migrating along the endothelial cell. Scale bar = 50 μm. b and c from Lugassy et al. (2013a and 2013b). (d) Chick chorioallantoic membrane (CAM) assay. Spreading of green fluorescent protein melanoma cells along vessels. Scale bar = 1 mm. (e) Histological confirmation of melanoma angiotropism, indicating pericytic mimicry. Scale bar = 50 μm. d and e reprinted with permission from Lugassy et al. (2007). (f) Macroscopically visible melanoma pigment spreading along dermal blood vessels in a murine melanoma model over 25 mm. Scale bar = 1 mm. (g and h) Microscopic angiotropism, indicating pericytic mimicry. (i) Similar angiotropism in a human primary melanoma. Scale bar = 50 μm. f–i reprinted with permission from Bald et al. (2014). (j) Diagram of pericytic mimicry.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2015 The Authors Terms and Conditions

3. Figure 2
Graphical abstract illustrating how neutrophilic inflammation can promote angiotropism, pericyitic mimicry, and metastasis of melanoma. Neutrophilic inflammation shifts melanoma cells toward a migratory phenotype, promotes melanoma cell spreading in a pericyte location along abluminal vascular surfaces, and enhances the metastatic capacity. Further investigations are needed to clarify the relative importance of extravascular migratory metastasis versus intravascular dissemination in melanoma progression.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2015 The Authors Terms and Conditions