1. Volume 120, Issue 6, Pages 1412-1419 (May 2001)
Expression of the duodenal iron transporters divalent-metal transporter 1 and ferroportin 1 in iron deficiency and iron overload 
Heinz Zoller, Günter Weiss, Igor Theurl, Robert O. Koch, Wolfgang Vogel 
Gastroenterology 
Volume 120, Issue 6, Pages (May 2001)
DOI: /gast
Copyright © 2001 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Quantification of duodenal DMT1 cDNA expression by real-time PCR. Results of TaqMan real-time PCR of 5 representative patients are shown. Fluorescence activity (ΔRn) is blotted vs. cycle number. (●) Normal patient; (▿) iron-deficient patient; (♦) C282Y patient; (○) non-HFE HH patient; (×) patient with secondary iron overload. For details, see Materials and Methods.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

3. Fig. 2
DMT1 and FP1 cDNA levels in duodenal biopsy specimens from patients with disorders of iron metabolism. (A) DMT1/GAPDH cDNA ratio in logarithmic scale, median (horizontal line), 75% confidence interval (box), and minimal and maximal values (vertical line) in the respective patient groups. (B) Logarithmic FP1/GAPDH cDNA ratio (median, 75% confidence interval, and minimal and maximal values).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

4. Fig. 3
DMT1 protein expression in duodenal biopsy specimen Western blot analysis for DMT1 of protein extracts from duodenal biopsy specimens. A representative example of each patient group is shown. The 60-kilodalton band corresponds to the DMT1 protein. Relative intensities were estimated from densitometric scanning of Western membranes and are expressed as percentages of control (100%; means ± SD for 4 experiments). *P < 0.05 compared with control.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Distribution of DMT1 protein in patients with various iron disorders. Immunohistochemical staining for DMT1 of duodenal biopsy specimen from 5 representative patients is shown. Staining is most prominent on the apical membrane of duodenal enterocytes. (A) Patient with normal serum iron parameters; (B) iron-deficient patient; (C) HFE-HH (C282Y homozygous); (D) non-HFE HH; (E) secondary iron overload.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Distribution of FP1 in biopsy specimens from normal, iron-deficient, and iron-overloaded patients. Representative cryosections of duodenal biopsy specimens from (A) a normal patient, (B) an iron-deficient patient, and (C) a patient with HFE HH are shown. The staining is more prominent in anemic and iron-overloaded patients than in the control patient, and the highest intensity is in the basolateral membrane.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

7. Fig. 6
Association between duodenal DMT1 and FP1 mRNA expression. Correlation between DMT1/GAPDH and FP1/GAPDH ratios is shown in all patients investigated independent of underlying disease (all single values; the regression line and 95% confidence intervals are plotted).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions