1. A novel approach with magnetic resonance imaging used for the detection of lung allograft rejection 
Shinichi Kanno, MD, PhDa, Paul C. Lee, MDb, Stephen J. Dodd, PhDa, Mangay Williams, PhDa, Bartley P. Griffith, MDb, Chien Ho, PhDa 
The Journal of Thoracic and Cardiovascular Surgery 
Volume 120, Issue 5, Pages (November 2000)
DOI: /mtc
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

2. Fig. 1
Gradient-echo MR images of macrophage samples in vitro. Cells were prepared as described in the “Methods” section. Cell concentration of the samples was 2.0 × 106 cells/mL of 5% gelatin in A and B and 0.5 × 106 cells/mL gelatin in C and D. Cells treated with USPIO particles are shown in A and C and cells not treated with USPIO particles are shown in B and D.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

3. Fig. 2
A new heterotopic lung transplantation model suited for MR signal detection. An en bloc heart and right lung were transplanted to the groin area of the recipient. The gross view of the surgical field is shown in the left panel. The transplanted heart is indicated by the arrow and the transplanted lung is indicated by the arrowhead. The aorta of the graft was connected to the abdominal aorta of the recipient. The transplanted grafts were isolated from abdominal organs by the abdominal wall and put in the subcutaneous tissue. A representative MR image of the allotransplant on postoperative day 4 in the coronal plane is shown in the right panel. The area encompassed by the dotted line is the transplanted graft. The lung is indicated by the arrowhead.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

4. Fig. 3
MR images showing the effect of USPIO particle infusion in an allotransplant. MR images of the coronal view of the graft are shown in A and B, and the sagittal view of the graft is shown in C and D. The MR images of the graft on postoperative day 4 before USPIO infusion are at A and C, and the images taken 24 hours after infusion are at B and D. The lung is indicated by the arrow in each panel. Representative images are shown.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

5. Fig. 4
MR images showing the effect of USPIO particle infusion in an isograft. MR images of the coronal view of the graft are shown. The MR images of the isograft on postoperative day 4 before USPIO infusion are at A and C, and the images taken 24 hours after infusion are at B and D. The lung is indicated by the arrow in each panel. Representative images are shown.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

6. Fig. 5
MR images showing the effect of USPIO particle infusion in the allotransplant treated with cyclosporine A (CsA). MR images of the coronal view of the graft are shown. The MR images of the transplanted lung on postoperative day 4 before USPIO infusion are at A and C, and the images taken 24 hours after infusion are at B and D. The lung is indicated by the arrow in each panel. Representative images are shown.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

7. Fig. 6
Summary of the measurements of MR signal intensity in transplanted lungs. MR signal intensity was measured at 5 different points of the horizontal image of each transplanted lung. Data are indicated as mean ± standard deviation of the mean, shown as arbitrary units (n = 30, 5 points/rat). Allografts (solid square) showed significantly lower MR intensity compared with isografts (open square) at every time point after USPIO infusion. Allografts with CsA treatment (diamond) also showed significantly lower MR intensity compared with isografts. There was also a significant difference between allografts with or without CsA treatment.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

8. Fig. 7
Immunohistologic counts of CD3+ or ED1+ cells. The CD3+ or ED1+ cells were counted in at least 10 fields per section per rat (n = 6 in each group). The fields were randomly chosen and counting was performed in a blinded manner at a magnification of ×100. Data are presented as mean ± standard deviation of the mean.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

9. Fig. 8
Histologic and immunohistochemical analyses of an allograft. Perls' Prussian blue reaction shows the distribution of the iron-containing cells (A). B and C show ED1+ or CD3+ cells, respectively. Comparing B with A, the distribution of the iron-containing cells completely correlates with the presence of ED1+ cells, whereas, comparing C with A, the distribution of CD3+ cells does not correlate with that of iron staining. Figures were at a magnification of ×100.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

10. Fig. 9
A high-power field image of Perls' Prussian blue iron staining of an allograft 5 days after transplantation. The spots of iron particles are found in the cytoplasm of macrophages. Magnification was ×400.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc )
Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions