1. First breeding works at Lycium sp in UASMV Bucharest
Curelaru Vlad Constantin
Assoc. Prof. Dr. Adrian Asanică
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania

2. Introduction
The original habitat of the goji plant, often referred to as the wolfberry, is to date unknown. The region of origin is proposed to be somewhere in the south-eastern parts of Europe or the south-western region of Asia. Nowadays this plant is considered a highly valuable plant in medicine, health, treatments, horticulture production and economic investment due to the prize of the berries
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania

3. A Vitamins C E Antioxidants Fiber Beta-carotene
The fruit of Lycium barbarum have a variety of pharmacologic functions, life extending capabilities, anti-depressant and anti-bacterial properties.

4. Materials and Methods Choice of parents, buds, flowers
Hybrid fruit
Choice of parents, buds, flowers
Flower emasculation
Izolation of emasculated flowers
Harvest of flower pollen
Pollen conservation
(if need)
Breeding

5. Emasculation: removal of all anthers or stamens to prevent self-pollination.

6. Isolation of flowers: To protect them from foreign pollenm we cover the flowers with a paper bag.

7. Directly by touching with flowers With previous collected pollen
BREEDING - crossing
Directly by touching with flowers
With previous collected pollen

8. Hybrid fruit with hybrid seeds

9. The seedling and emerging of hybrid seeds

10. Results and discussions
We have 6 biotypes of Lycium sp. as parents:
Matern and Patern use B1 B2
410
Only patern use AS C9
BUA
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania

11. First series: extracted pollen
Hybrid combination
Emasculated flowers
Pollinated flowers
Related fruits
Mature fruit
% hybrid fruit
♀B1 x ♂B2 20 7 1 5
♀B1 x ♂410 4 1.
♀B2 x ♂B1
♀B2 x ♂410 9
♀410 x ♂B1 15 8
♀410 x ♂B2
6,6 13
30,7 6
16,6 2. 21 3
42,8
First series: extracted pollen
Second series: pollination by touching directly with flowers

12. Series
Hybrid combination
Emasculated flowers
Pollinated flowers
Related fruits
Mature fruit
% hybrid fruit
♀B1 x ♂B2 20 8 6 30
♀B1 x ♂410 16 5 1
♀B1 x ♂Bua
♀B1 x ♂C9 2
♀B2 x ♂B1 40
♀B2 x ♂410 15 9 3.
♀B2 x ♂AS 10
♀B2 x ♂Bua
♀B2 x ♂C9
♀410 x ♂B1 7
♀410 x ♂B2 4
♀410 x ♂AS
100
♀410 x ♂Bua 50
It can be noticed that the best results in the third series was with 410 as maternal genitor

13. Poor results with extracted pollen.
Series
Hybrid combination
Number of seeds
Number of emerged plants
% emerged plants
♀B1 x ♂B2 8
♀B1 x ♂410 6 1.
♀B2 x ♂B1 25
♀B2 x ♂410
♀410 x ♂B1
♀410 x ♂B2 1 66 5
7,5 32 22
68,75 2. 57 38
66,66
Poor results with extracted pollen.

14. Series
Hybrid combination
Number of seeds
Number of emerged plants
% emerged plants
♀B1 x ♂B2
100 9
9,0
♀B1 x ♂410 6
♀B1 x ♂Bua 4
♀B1 x ♂C9
♀B2 x ♂B1
115 1
0,9
♀B2 x ♂410 3.
♀B2 x ♂AS 26
15,4
♀B2 x ♂Bua
♀B2 x ♂C9
♀410 x ♂B1 35
♀410 x ♂B2 24
8.6
♀410 x ♂AS
15.4
♀410 x ♂Bua
In the 3rd series of breeding only the combination with AS variety as paternal genitor had satisfactory results.

15. Conclusions and recommendations
Very good results after using the direct flower touch pollination method.
The pollen had a very high viability, around 98% to all studied biotypes.
The capacity of pollen germination was between 9,43% for biotype B1, 10,45% for biotype 410 and 15,38% for biotype B2.
The calendar for the start of work must be earlier, immediately after the binding for the first series of flowers, first of all due to a period of growing and fructification of hybrids by the beginning of October and also at the beginning of the fructification period (June-July) the plants present a optimal health status, the hybrid fruits growing normally.
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania

16. for your attention and don’t ask too much :)
Thank you
for your attention and don’t ask too much :)
International Student Symposium "Hortus Academicus“ April 27, Bucharest, Romania