1. Changes in Cholesterol Level Alter Integrin Sequestration in Raft-Mimicking Lipid Mixtures 
Yifan Ge, Jiayun Gao, Rainer Jordan, Christoph A. Naumann 
Biophysical Journal 
Volume 114, Issue 1, Pages (January 2018)
DOI: /j.bpj
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2. Figure 1
Representative EPI micrographs of NBD-DHPE distribution in a polymer-tethered lipid bilayer that contains equimolar concentrations of DOPC and DPPC and varying CHOL molar concentration of 25, 28, 33, 35, 37, and 40 mol%.
Biophysical Journal  , DOI: ( /j.bpj )
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3. Figure 2
Analysis of domain-specific TRITC-DHPE diffusivity (A) and Eraft data of NBD-DHPE distribution (B) suggest that variation in membrane CHOL between 25 and 37 mol% CHOL alters lipid packing differences between lo and ld domains of a polymer-tethered lipid bilayer containing DOPC/DPPC/CHOL mixtures (1:1 DOPC/DPPC molar ratio); p < Error bars represent standard deviation of data sets from 15 different bilayer regions (out of three samples).
Biophysical Journal  , DOI: ( /j.bpj )
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4. Figure 3
Representative CS-XY scans (raw data) of NBD-DHPE (A–C and G–I) and Alexa 555 MAb-tagged αvβ3-integrin distributions (D–F and J–L) in DOPC/DPPC/CHOL mixtures containing equimolar amounts of DOPC and DPPC and varying CHOL molar concentrations of 25, 33, and 37 mol%, as obtained through the NBD and Alexa 555 channels of the confocal detection system (p < 0.01). Separate data sets are presented for αvβ3 (A–F) and αvβ3 + VN (G–L). Quantification of the CS-XY data in terms of the parameter Eraft (see Eq. 1) demonstrates the profound influence of CHOL on the sequestration of αvβ3 before and after VN addition (M); p < 0.01 (box size; CS-XY scan: 12 × 12 μm2, pixel size: 0.5 μm). Error bars in (M) represent SD of Eraft acquisitions from 15 different bilayer regions (out of three samples). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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5. Figure 4
Representative time scans of confocal fluorescence intensity (A) and corresponding PCH curves (B) of Alexa 555 MAb-labeled αvβ3 in lo and ld domains in a polymer-tethered lipid bilayer comprised of an equimolar ratio of DOPC and DPPC and 33 mol% CHOL. Parameter Xdimer from PCH analysis demonstrates that changes in CHOL content do not alter the predominantly monomeric nature of integrins in the bilayer (C) (p < 0.01). Data in (B) and (C) are based on 50 independent data sets for each lipid composition. Errors bars represent standard deviation.
Biophysical Journal  , DOI: ( /j.bpj )
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6. Figure 5
Populations of αvβ3 and uPAR, Xmigrate (defined by Eq. 2), in a polymer-tethered lipid bilayer, which alter their phase preference in a ternary DOPC/DPPC/CHOL mixture in response to a change in membrane CHOL from 33 to 37 mol%; p < Data are based on 15 independent data sets of Eraft at 33 and 37 mol% CHOL. Errors bars represent corresponding standard deviation.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2017 Biophysical Society Terms and Conditions