1. Tissue Western analysis of purified monospecific antibodies.
Tissue Western analysis of purified monospecific antibodies. Total protein Western blots of a number of antibodies using protein extracts from human cell lines (RT-4, EFO-21, and A-431) and tissues (liver and tonsil) are shown. The filled arrow indicates the size of the expected protein as determined from the genome sequence. a, antibody HPR generated toward the 61-kDa cleavage stimulation factor subunit encoded by the CSTF2 gene located on the X chromosome. b, antibody HPR raised against the 22-kDa BH3-interacting domain death agonist (BID) transcribed from chromosome 22. c, HPR recognizing the 126-kDa WD repeat and high mobility group box DNA-binding protein 1 encoded by the WDHD1gene on chromosome 14. d, HPR binding to an unknown 57-kDa protein. e, HPR generated toward the heat shock-related 70-kDa protein 2 gene HSPA2 located on chromosome 14. f, HPR00129 designed toward the snRNA-activating protein complex 43-kDa subunit. MWM, molecular weight marker.
Mathias Uhlén et al. Mol Cell Proteomics 2005;4:
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