1. Volume 26, Issue 1, Pages 280-288 (January 2018)
Absence of Replication-Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products 
Kenneth Cornetta, Lisa Duffy, Cameron J. Turtle, Michael Jensen, Stephen Forman, Gwendolyn Binder-Scholl, Terry Fry, Anne Chew, David G. Maloney, Carl H. June 
Molecular Therapy 
Volume 26, Issue 1, Pages (January 2018)
DOI: /j.ymthe
Copyright © 2017 The American Society of Gene and Cell Therapy Terms and Conditions

2. Figure 1 Schematic Representation of the RCL Assay
T cell products are incubated with C cells at a ratio of 1:5. Over half of test articles submitted contained approximately 1 × 108 T cells and were divided into twenty-five 300-cm2 flasks. Cultures were passed a minimum of 5 passages using increasingly smaller vessels. After 3 weeks, cells were placed in fresh media, and conditioned media were harvested after 24 hr. Media from all amplification cultures are pooled, and two aliquots are then incubated with naive C cells. After 7 days, culture media were analyzed for p24 antigen by ELISA and either the psi-gag PCR assay or PERT. The figure depicts amplification of a low-titer RCL present in the test article.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2017 The American Society of Gene and Cell Therapy Terms and Conditions