1. Microbiome contribution and metabolite intervention in post-dieting weight-regain
MSc. Lenka Dohnalová, July 2017, Munich

2. Yo-Yo effect

3. Yo-Yo effect

4. Long-term weight loss maintenance
(1949): 100 obese individual → 2 % lost weight for 5 years
(2005): 500 individuals from US → 20 % manage to loss about 10 % weight for at least 1 year
● without physical activity
▪ with physical activity
Wing and Phelan, 2005; Tate et al., 2007; Hall and Jordan, 2008

5. Factors affecting weight regain
genetics vs non-genetic factors
Levy et al., 2017

6. Factors affecting weight regain
genetics vs non-genetic factors - 90 % of genes - metabolic pathways - key role in immunity - easy to transfer
Levy et al., 2017

7. Factors affecting weight regain
genetics vs non-genetic factors
Levy et al., 2017

8. Microbiota metabolites in immunity
Angelakis et al., 2012; Levy et al., 2017

9. HFD-induced obesity

10. HFD-induced obesity

11. HFD-induced obesity

12. Metabolic parameters at weight-regain

13. Microbiome composition alterations analysis

14. Post-dieting persistent microbiome
45 % pre-obese OTUs recovered
α-diversity decrised in HFD

15. Post-dieting persistent metagenome
Isoflavonoid biosynthesis
Steroid biosynthesis
Biosynthesis of unsaturated FA
N-glycan biosynthesis
DTT degradation
Flagellar assembly

16. Post-dieting persistent metabolic pathways

17. Antibiotics abolish the post-obesity microbiome signature

18. Antibiotics abolish the post-obesity microbiome signature

19. Microbiome transplantation ameliorates post-dieting weight

20. Microbiome transplantation ameliorates post-dieting weight

21. Microbiome transplantation ameliorates post-dieting weight

22. Microbiome transfer to GF affecting post-dieting weight regain

23. Metabolite contribution to post-dieting weight regain

24. Metabolite contribution to post-dieting weight regain

25. Flavonoid treatment to prevent post-dieting weight regain

26. Flavonoid treatment to prevent post-dieting weight regain

27. Flavonoid treatment to prevent post-dieting weight regain

28. Mechanism of flavonoid treatment in weight regain prevention
BAT (brown adipose tissue) → thermogenesis regulation
→ flavonoids induce UCP1 (uncoupling protein 1)

29. Mechanism of flavonoid treatment in weight regain prevention
BAT (brown adipose tissue) → thermogenesis regulation
→ flavonoids induce UCP1 (uncoupling protein 1)
→ HFD → to ↓ flavonoids pool
→ ↑ expression of ucp1 → ↓ E expenditure

30. Aknowledgement