1. A
CYP7A1
CYP7A1
1.2
1.2
AICAR (2 mM)
1.0
1.0
0.8 *
0.8
mRNA expression
0.6
mRNA expression *
0.6
0.4
0.4 * *
0.2
0.2
AICAR (mM) - 1 2 0h 2h 6h
24h B
CYP27A1
CYP27A1
1.2
1.4
AICAR (2 mM)
1.0
1.2
1.0
0.8
0.8
mRNA expression
0.6
mRNA expression
0.6
0.4
0.4
0.2
0.2
AICAR (mM) - 1 2 0h 2h 6h
24h C.
HNF4
HNF4
1.2
1.4
1.2
1.0
1.0
0.8
mRNA expression
0.8
mRNA expression
0.6
0.6
0.4
0.4
0.2
0.2
AdGFP
AdAMPKα2
Ad DN-AMPK
AICAR(mM) - 1 2
Supplemental Fig 1. HepG2 cells were treated with AICAR for time periods and doses indicated, or infected with Ad-GFP, Ad-AMPKα2 or Ad-DN-AMPK at an MOI of ~ 10 for 24 hr. mRNA expressions were determined by real-time PCR in triplicates and expressed as mean ± S.D.

2. ChREBP mRNA expression
Sicon siACL siAceCS1
5.5 mM
27.7 mM
ChREBP 1 2 3 4 5 6 *
Supplemental Fig 2. HepG2 cells were transfected with siCon, siACL or siAceCS1 and treated with 5.5 or 27.5 mM glucose for 24 hr as described in Materials and Methods. The mRNA expression levels were determined by real-time PCR.