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R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E

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Presentation on theme: "R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E"— Presentation transcript:

1 Rapid detection of toxigenic Clostridium difficile strains by a nested PCR of the toxin B gene 
R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E. Bouza  Clinical Microbiology and Infection  Volume 3, Issue 1, Pages (February 1997) DOI: /j tb00271.x Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

2 Fig. 1 Amplification patterns of the assayed isolates. Lanes 1 and 10, molecular weight markers; lane 2, amplification band corresponding to C. difficile ATCC 9689; lane 3, amplification of a non-toxigenic C. difficile clinical isolate (no. 9415) DNA; lanes 4, 5 and 6, amplification of toxigenic C. difficile clinical isolates (numbers 9430, 9456 and 9490) DNAs (the first amplification product can also be seen in lane 6); lanes 7, 8 and 9, amplification of C. sordellii, C. ramosum and C. perfringens DNAs respectively. Clinical Microbiology and Infection 1997 3, DOI: ( /j tb00271.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions


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