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Figure 1. autoMLST workflow depicting placement and de novo mode

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Presentation on theme: "Figure 1. autoMLST workflow depicting placement and de novo mode"— Presentation transcript:

1 Figure 1. autoMLST workflow depicting placement and de novo mode
Figure 1. autoMLST workflow depicting placement and de novo mode. Estimated ANI values with reference genomes are found ... Figure 1. autoMLST workflow depicting placement and de novo mode. Estimated ANI values with reference genomes are found which is used for organism selection. This set is then screened for single copy genes present in every genome and prioritized based on MLSA criteria. Multiple sequence alignments are then obtained and trimmed. Final maximum-likelihood inference is calculated depending on the options and mode used. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, gkz282, The content of this slide may be subject to copyright: please see the slide notes for details.

2 Figure 2. Tree visualization provides options to toggle branch lengths, zoom, search and color the final tree. (A) ANI ... Figure 2. Tree visualization provides options to toggle branch lengths, zoom, search and color the final tree. (A) ANI group coloring. (B) Secondary metabolite coloring. (C) Sortable table of ANI values with search function. (C) Export functions to download trees, alignments, and supporting information. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, gkz282, The content of this slide may be subject to copyright: please see the slide notes for details.

3 Figure 3. Histograms of monophyletic scoring of ANI clans at three thresholds: 97%, 95% and 90% ANI. (A) Concatenated ... Figure 3. Histograms of monophyletic scoring of ANI clans at three thresholds: 97%, 95% and 90% ANI. (A) Concatenated workflow. (B) Coalescent workflow. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, gkz282, The content of this slide may be subject to copyright: please see the slide notes for details.

4 Figure 4. Comparison of trees generated automatically with autoMLST (left) a manual MLSA (right) provided by Dr Adamek ... Figure 4. Comparison of trees generated automatically with autoMLST (left) a manual MLSA (right) provided by Dr Adamek (46). Groups defined in this study are indicated using the same color scheme and labels as in Adamek et al. Comparison was made using the tanglegram algorithm in dendroscope (51). Further details can be seen in the Supplementary Figures S4 and S5. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact Nucleic Acids Res, gkz282, The content of this slide may be subject to copyright: please see the slide notes for details.

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