1. Identification of diabetes- and obesity-associated proteomic changes in human spermatozoa by difference gel electrophoresis 
Thomas M Kriegel, Falk Heidenreich, Karina Kettner, Theresia Pursche, Bernard Hoflack, Sonja Grunewald, Kerstin Poenicke, Hans- Juergen Glander, Uwe Paasch 
Reproductive BioMedicine Online 
Volume 19, Issue 5, Pages (November 2009)
DOI: /j.rbmo
Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

2. Figure 1
Overall experimental design for the identification of differentially expressed. Sperm cell proteins of non-diabetic obese and type 1 diabetic individuals by difference gel electrophoresis (DIGE).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

3. Figure 2
Difference gel electrophoresis (DIGE) images of CyDye-labelled human sperm proteomes of (A) type 1 diabetic, (B) non-diabetic obese, and (C) slim clinically healthy fertile individuals. (D) shows the image of the internal standard. The arrows in A and B indicate the position of sperm proteins exhibiting significantly increased (blue) or decreased (red) average abundance ratios (P⩽0.05). Corresponding gel discs were excised and subjected to tryptic proteolysis and mass spectrometric peptide identification. The positions of the identified differentially expressed proteins are also indicated in (C). For overview reasons, the positions of a limited number of additional sperm proteins are indicated (D). The identified proteins are annotated by their gene names according to the NCBInr protein database (for full protein names, see Tables 2–4).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions