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Volume 26, Issue 13, Pages 1677-1687 (July 2016)
Rewiring of the Ppr1 Zinc Cluster Transcription Factor from Purine Catabolism to Pyrimidine Biogenesis in the Saccharomycetaceae Walters Aji Tebung, Baharul I. Choudhury, Faiza Tebbji, Joachim Morschhäuser, Malcolm Whiteway Current Biology Volume 26, Issue 13, Pages (July 2016) DOI: /j.cub Copyright © 2016 Elsevier Ltd Terms and Conditions
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Figure 1 C. albicans Ppr1 Aligned with S. cerevisiae Ppr1
The left shaded alignment area has 38.10% identity and includes the zinc cluster domain. The larger shaded alignment area to the right has 40.73% identity. Current Biology , DOI: ( /j.cub ) Copyright © 2016 Elsevier Ltd Terms and Conditions
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Figure 2 Purine Catabolism Pathway, Ppr1 Motif Occurrence in the Promoters of De Novo Pyrimidine Biosynthesis and Purine Catabolism Genes, and the Presence or Absence of DAL82 Genes across the Ascomycetes (A) Ppr1 motif occurrence in the promoters of de novo pyrimidine biosynthesis (URA) and purine catabolism (includes DAL) genes, and the presence or absence of DAL82 genes across the Ascomycetes. Dark blue boxes with motif logo represent the Ppr1 motif; gray boxes indicate gene presence; white boxes indicate gene absence; and blue boxes imply that the sequence is not available in databases. A, Dal82 absent; P, Dal82 present. The phylogenetic tree is based on Wang et al. [29] and the Fungal Orthogroups Repository ( (B) Purine catabolism pathway [30]. Blue enzymes: present in C. albicans and regulated by Ppr1 but missing in S. cerevisiae; green enzymes: regulated by both Ppr1 in C. albicans and Dal82 in S. cerevisiae; red enzymes: regulated by Ppr1 in C. albicans and present in S. cerevisiae, but not regulated by Dal82 in S. cerevisiae. Dashed lines represent non-enzymatic reactions. Current Biology , DOI: ( /j.cub ) Copyright © 2016 Elsevier Ltd Terms and Conditions
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Figure 3 Occurrence of Ppr1 and Non-Ppr1 Motifs across the Ascomycetes Fungi The Ppr1 motif (green boxes) is present only throughout Saccharomyces species, whereas an alternative GryTkGwnTGGT-like motif (yellow boxes) is present across non-Saccharomyces species. The presence and absence of a promoter sequence are represented by gray and white boxes, respectively. The motif scan was performed at the 90% threshold using the software package MotifLab. The phylogenetic tree is based on Wang et al. [29] and the Fungal Orthogroups Repository ( Current Biology , DOI: ( /j.cub ) Copyright © 2016 Elsevier Ltd Terms and Conditions
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Figure 4 Ppr1 DNA Binding Sequence in C. albicans and S. cerevisiae
(A) C. albicans Ppr1 DNA binding motif based on the top 50 Ppr1 binding sites from ChIP-chip. ChIP-chip-identified sequences used to identify the C. albicans Ppr1 DNA binding motif are presented in Table S2. (B) S. cerevisiae Ppr1 DNA binding motif [27]. Current Biology , DOI: ( /j.cub ) Copyright © 2016 Elsevier Ltd Terms and Conditions
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Figure 5 Allantoin, Ammonium Sulfate, and Urea Utilization Assays of a C. albicans ppr1 Null Mutant Strain, Ppr1 Gain-of-Function Mutant Strain, and C. albicans Wild-Type Strain SC5314 (A) Growth curve of the ppr1 null mutant (ΔΔppr1) strain, Ppr1 gain-of-function mutant (Ppr1-GOF) strain, and SC5314 (WT, wild-type) strain in yeast carbon base (YCB) media with allantoin as the sole nitrogen source. (B) Growth curve of the ppr1 null mutant strain, Ppr1 gain-of-function mutant strain, and SC5314 strain in YCB media with ammonium sulfate as the sole nitrogen source. (C) Growth curve of the ppr1 null mutant strain, Ppr1 gain-of-function mutant strain, and SC5314 strain in YCB media with urea as the sole nitrogen source. Error bars are based on the SD of the seven replicates of each data point reading taken every 2 hr. Primers used for transformation in this study are presented in Table S1. Current Biology , DOI: ( /j.cub ) Copyright © 2016 Elsevier Ltd Terms and Conditions
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