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W. Hryniewicz, K. Szczypa, M. Bronk, A. Samet, A. Hellmann, K

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Presentation on theme: "W. Hryniewicz, K. Szczypa, M. Bronk, A. Samet, A. Hellmann, K"— Presentation transcript:

1 First report of vancomycin-resistant Enterococcus faecium isolated in Poland 
W. Hryniewicz, K. Szczypa, M. Bronk, A. Samet, A. Hellmann, K. Trzcinski  Clinical Microbiology and Infection  Volume 5, Issue 8, Pages (August 1999) DOI: /j tb00181.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

2 Figure 1 RAPD products obtained in PCR reactions using ERIC1, AP4 and ERIC1/AP4 primers. Molecular size markers are indicated (S1, λ/BstEII; S2, pBR 322/MspI). Lane 1, isolate 1 (1639); lane 2, isolate 2 (1640); lane 3, isolate 3 (1641). Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00181.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

3 Figure 2 Pulsed-field gel electrophoresis of BilII-digested chromosomal DNA from E. faecium isolates. Molecular masses (in kilobases) were obtained from bacteriophage DNA concatamers (S). Lane 1, isolate 1 (1639); lane 2, isolate 2 (1640); lane 3, isolate 3 (1641). Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00181.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

4 Figure 3 Products of specific PCR amplification of vanA gene. Molecular size markers are indicated. (S1, λ/βstΕII; S2, pBR 322/MspI). Lane 1, glycopeptide-susceptible E. faecium BM4147–1; lane 2, glycopeptide-resistant E. faecium BM4147; lane 3, isolate 1 (1639); lane 4, isolate 2 (1640); lane 5, isolate 3 (1641). Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00181.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions


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