1. B7.2−/− Mature Dendritic Cells Generate T-Helper 2 and Regulatory T Donor Cells in Fetal Mice after In Utero Allogeneic Bone Marrow Transplantation 
Swati Bhattacharyya, Morton J. Cowan 
Biology of Blood and Marrow Transplantation 
Volume 11, Issue 9, Pages (September 2005)
DOI: /j.bbmt
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
Proliferation of allogeneic (BALB/c) CD3+ splenocytes in response to varying numbers of B6 mDCs from WT and knockout mice. A, Mature DCs from WT B6 donors. B, Mature DCs from B7.1−/− B6 donors. C, Mature DCs from B7.2−/− B6 donors.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
Fluorescence-activated cell-sorting analysis of donor H2Kb+CD4+ T cells in blood 6 weeks after IUT with lin− BM plus B7.2−/− mDCs. A, Dot-plot analysis of CD3 versus CD4 of H2Kb+ gated cells. BALB/c and B6 blood were used as negative and positive controls, respectively. B, Cytokine expression (PE-conjugated interferon γ, IL-2, IL-10, and IL-4) in H2Kb+CD4+ splenocytes from an IUT recipient. C, CD25 expression in H2Kb+CD4+ splenocytes after IUT.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

4. Figure 3
Inhibition of the MLR by CD4+CD25+ and CD4+CD25− T-regulatory cells. CD4+CD25+ and CD4+CD25− T cells were evaluated as regulatory cells to inhibit the allogeneic MLR between BALB/c splenocyte responders and irradiated B6 splenocyte stimulators. The experiment was repeated twice. A, Donor CD4+CD25− versus CD4+CD25+ T-cell fractions were cocultured in the MLR. B, Effect of anticytokine antibodies (anti–IL-10, anti–IL-4, and anti–TGF-β) on in vitro proliferation of allogeneic (BALB/c) CD3+ splenocytes when stimulated with donor H2Kb+CD4+CD25− T cells. C, Effect of anticytokine antibodies (anti–IL-10, anti–IL-4, and anti–TGF-β) on in vitro proliferation of allogeneic (BALB/c) CD3+ splenocytes when stimulated with donor H2Kb+CD4+CD25+ T cells.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

5. Figure 4
Characterization of donor cell engraftment in pooled tissues (spleen and thymus) of IUT recipients of mDCs from B7.2−/− B6 and WT lin− BM from B6 donors. The percentage and subtype of donor cells were evaluated by 5-color flow cytometry analysis and sorting. A, Donor (H2Kb+) cells in spleen at the time of harvest: dot plot shows immunophenotyping of donor (H2Kb+) cells in spleen, CD4-APC, and CD25/peridinin chlorophyll protein (PerCP)/Cy5. B, Further characterization of donor (H2Kb+CD4+CD25+) cells in spleen by immunophenotyping for CTLA-4/PE. C, Donor (H2Kb+) cells in the thymus at the time of harvest showing immunophenotyping of donor (H2Kb+) cells in the thymus for CD4-APC and CD25-PerCP-Cy5. D, Further characterization of donor (H2Kb+CD4+CD25+) cells in spleen by immunophenotyping for CTLA-4/PE. The donor cells in the thymus were also analyzed for dendritic cell and T-cell phenotype. E, Donor (H2Kb+) cells in the thymus at the time of harvest. The dot plot shows CD3-APC versus H2Kb-FITC. F, Thymic CD3-APC gated cells plotted for CD4-PE versus CD8-APC-Cy7. G, Thymic donor dendritic cells in which H2Kb+ cells were evaluated for CD11c-PerCP-Cy5 versus MHC II/PE. H, Cells displaying high CD11c and MHC II were gated and plotted for CD80-APC and CD86-APC-Cy7.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

6. Figure 5
Donor cell engraftment in IUT recipients that underwent subsequent megadose transplantation. The percentage and subtype of donor cells were evaluated by flow cytometry and PCR. A, Donor (H2Kb+) cells in blood 6 weeks after mega-BMT in IUT recipients with lin− BM plus B7.2−/− mDCs. B, Lineage analysis of donor (H2Kb+) cells in blood 6 weeks after mega-BMT in IUT recipients with lin− BM and B7.1−/− mDCs. C, PCR-amplified H2Kb products in WT B6 control splenocytes (lane 1) and in thymus (lanes 2 and 7), spleen (lanes 3 and 8), blood (lanes 4 and 9), and bone marrow (lanes 5 and 10) of IUT recipients of lin− BM plus B7.1−/− mDCs (lanes 2–5) and lin− BM plus B7.2−/− mDCs (lanes 7–10).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions