1. Effects of the FSH receptor gene polymorphism p
Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells 
Verena Nordhoff, Barbara Sonntag, Dominik von Tils, Martin Götte, Andreas N. Schüring, Jörg Gromoll, Klaus Redmann, Livio Casarini, Manuela Simoni 
Reproductive BioMedicine Online 
Volume 23, Issue 2, Pages (August 2011)
DOI: /j.rbmo
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2. Figure 1
Basal and stimulated oestradiol (A) and progesterone (B) production of day-2 GC cultures with the NN and SS genotypes. Applying two-way-ANOVA, oestradiol, but not progesterone, concentration was significantly different with respect to FSHR variant (oestradiol, P=0.045) and irrespective of stimulatory treatment. Asterisk=significant differences between individual groups; NS=not statistically significant.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Representative time course of FSH receptor gene (A–C), inhibin (D–F), oestradiol receptor α (G–I) and oestradiol receptor β (J–L) expression during prolonged culture of human granulosa cells of a heterozygous (NS) patient. GC were cultured for the indicated time points and processed for immunofluorescence microscopy. Green=investigated antigens, red=phalloidin counterstaining of the actin cytoskeleton. (M) Time-dependent re-appearance of FSHR in granulosa cells of a heterozygous (NS) patient measured by flow cytometry. Bar=50μm for panels A–L.
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4. Figure 3
Comparison of mean dose response curves following FSH stimulation between (A) NN and SS cAMP, (C) NN and SS progesterone and (E) NN and SS oestradiol following a non-linear fit in normalized semi-logarithmic distributions. Bars=standard deviations. Representation of (B) NN and SS cAMP ED50, (D) NN and SS progesterone ED50 and (F) NN and SS oestradiol ED50 with box and whisker plots. Data sets with abnormal response trends or inaccurate ED values were excluded from the analysis, no significant difference with Mann–Whitney U-test analysis was observed.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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