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Bacterial resistance of refrigerated and cryopreserved aortic allografts in an experimental virulent infection model  Pierre-Yves Litzler, MD, Pascale.

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Presentation on theme: "Bacterial resistance of refrigerated and cryopreserved aortic allografts in an experimental virulent infection model  Pierre-Yves Litzler, MD, Pascale."— Presentation transcript:

1 Bacterial resistance of refrigerated and cryopreserved aortic allografts in an experimental virulent infection model  Pierre-Yves Litzler, MD, Pascale Thomas, MD, Eric Danielou, MD, Julia Lucq, MD, Bertrand Jacques, MD, Noelle Frebourg, MD, Didier Plissonnier, MD, PhD, Dominique Bastit, MD, Josette Metayer, MD, Christophe Peillon, MD, Jacques Testart, MD, Jacques Watelet, MD  Journal of Vascular Surgery  Volume 29, Issue 6, Pages (June 1999) DOI: /S (99) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Time-kill curves of Staphylococcus aureus PR209, with cryopreservation or refrigerated medium. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Histologic appearance of an 8-day cryopreserved abdominal aorta. Cryopreservation preserved endothelium cells better, as evidenced by factor VIII antibody staining ( arrows) at original magnification of ×100. L, Lumen; I, intima; M, media. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Histologic appearance of an 8-day refrigerated abdominal aorta. Staining with factor VIII antibody at original magnification of ×100 shows the absence of the endothelium cell lining. L, Lumen; I, intima; M, media. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Histologic appearance of a refrigerated allograft after 4 weeks of implantation in infected sites. Hematoxylin-eosin stain at original magnification of ×40 shows intimal proliferation, including significant inflammatory infiltrates. L, Lumen; I, intima; M, media. Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Histologic aspect of a cryopreserved allograft after 4 weeks of implantation in infected sites. Factor VIII staining at original magnification of ×100 reveals endothelial cell recovery, with numerous plump cells (arrows). Journal of Vascular Surgery  , DOI: ( /S (99) ) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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